PDF DatasheetDiscontinued
Antibody (Suitable for clinical applications)
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
CD163 Information for Pathologists
Summary:
CD163 is a scavenger receptor that binds hemoglobin-haptoglobin complexes in plasma. The soluble form of the protein attenuates immune responses.
Common Uses By Pathologists:
CD163 staining is observed in histiocytic sarcoma. Monocytes and macrophages stain CD163+ in most healthy and disease samples.
| CD163 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 125.5 kDa | |
| Chromosomal Location | |
| p13.31 [chr: 12] [chr_start: 7470813] [chr_end: 7503893] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD163 |
| Entrez Gene ID | 9332 |
| RefSeq Protein Accession(s) | XP_016875720; NP_981961; NP_004235 |
| RefSeq mRNA Accession(s) | XR_002957389; NM_004244; NM_203416; XM_024449278; NM_001370146; NM_001370145; NR_163255 |
| RefSeq Genomic Accession(s) | NG_029826; NC_000012 |
| UniProt ID(s) | Q86VB7 |
| PharmGKB ID(s) | PA26190 |
| KEGG Gene ID(s) | hsa:9332 |
| General Description of CD163 . | |
| The CD163 molecule is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 protein is restricted in its expression to the monocytic/macrophage lineage. It is reported to be present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhans cells. In addition, multinucleated cells within inflammatory lesions are reported not to express CD163 protein. The protein is upregulated by glucocorticoids and downregulated by the immunosuppressant cyclosporin A and by phorbol esters. | |
There are no reviews yet.