PDF DatasheetHuman Anti-CD20 / MS4A1 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD20 / MS4A1 Antibody Clone: IGEL/773
Clone IGEL/773 Host and Isotype: Mouse IgG2a kappa
Anti-Human CD20 / MS4A1 Positive Control Sample: Daudi, Raji,, U266,, human lymphocytes. Lymph nodes, tonsils.
Cellular Localization of Antibody Predominantly cell surface with some cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MS4A1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD20 / MS4A1 Previously Observed Antibody Staining Patterns
| CD20 / MS4A1 General Information | |
|---|---|
| Alternate Names | |
| B-lymphocyte antigen CD20, CD20, Cluster of Differentiation 20 | |
| Molecular Weight | |
| 33-37kDa | |
| Chromosomal Location | |
| 11q12.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MS4A1 |
| Entrez Gene ID | 931 |
| Ensemble Gene ID | ENSG00000156738 |
| RefSeq Protein Accession(s) | NP_068769, NP_690605 |
| RefSeq mRNA Accession(s) | NM_152866, NM_021950 |
| RefSeq Genomic Accession(s) | NG_023388, NC_018922, NC_000011 |
| UniProt ID(s) | P11836, A0A024R507 |
| UniGene ID(s) | P11836, A0A024R507 |
| HGNC ID(s) | 7315 |
| Cosmic ID(s) | MS4A1 |
| KEGG Gene ID(s) | hsa:931 |
| PharmGKB ID(s) | PA31111 |
| General Description of CD20 / MS4A1. | |
| Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells, its expression is restricted to normal, neoplastic B-cells, being absent from all other leukocytes, tissues. CD20 is expressed by pre B-cells, persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia, malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood, lymphoid tissues, their derived lymphomas. In lymphoid tissue, germinal center blasts, B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type. | |
Limitations and Warranty
enQuire Bio’s CD20 / MS4A1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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