PDF DatasheetHuman Anti-CD3 Antibody Product Attributes
Species: Human
Cross Reactivity: chimpanzee
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: SK7
Clone SK7 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD3 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3 could be seen in. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD3 General Information | |
|---|---|
| Alternate Names | |
| Leu-4, T3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD3E |
| Entrez Gene ID | 916 |
| Ensemble Gene ID | ENSG00000198851 |
| RefSeq Protein Accession(s) | NP_000724 |
| RefSeq mRNA Accession(s) | NM_000733, |
| RefSeq Genomic Accession(s) | NC_018922, NG_007383, NC_000011 |
| UniProt ID(s) | P07766 |
| UniGene ID(s) | P07766 |
| HGNC ID(s) | 1674 |
| Cosmic ID(s) | CD3E |
| KEGG Gene ID(s) | hsa:916 |
| PharmGKB ID(s) | PA26216 |
| General Description of CD3. | |
| The SK7 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The SK7 antibody is a widely used phenotypic marker for human T cells. This antibody may induce T cell activation in the presence of monocytes. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3. Binding of clone SK7 can be blocked by an alternative Human Anti-CD3 clone, OKT3. Please choose the appropriate format for each application. | |
Selected References
McMichael AJ, Beverly PCL, Gilks W, et al, ed. Leukocyte Typing III: White Cell Differentiation Antigens. New York: Oxford University Press; 1987.Kaneoka H, Perez-Rojas G, Sasasuki T, Benike CJ and Engleman EG. 1983. J Immunol. 131: 158-164. (in vitro activation)Haringman JJ, Vinkenoog M, Gerlag DM, Smeets TJM, Zwinderman AH and Tak PP. 2005. Arthritis Res Ther. 7(4): R862-R867. (Immunohistochemistry – frozen tissue)Goval J-J, Greimers R, Boniver J and de Leval L. 2006. J Histochem Cytochem. 54(1): 75-84. (Immunofluorescence – frozen tissue)Wrann CD, Laue T, Hubner L, Kuhlmann S, Jacobs R, Goudeva L and Nave H. 2012. Am J Physiol Endocrinol Metab. 302(1): E108-E116. (Flow Cytometry)Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM and Varki A. 2010. J Immunol. 184(8): 4185-4195. (Flow Cytometry – Chimpanzee)
Limitations and Warranty
enQuire Bio’s Human Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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