PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Polyclonal
Anti-CD31 Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Placenta. Angiosarcoma
Cellular Localization of Antibody Polyclonal Staining: Cell Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues on human CD31 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CD31 Information for Pathologists
| CD31 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 82.5 kDa | |
| Chromosomal Location | |
| q23.3 [chr: 17] [chr_start: 64319415] [chr_end: 64413776] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PECAM1 |
| Entrez Gene ID | 5175 |
| RefSeq Protein Accession(s) | XP_005276939; NP_000433; XP_016880228; XP_011523192; XP_005276937; XP_005276938; XP_011523191; XP_016880227; XP_016880229 |
| RefSeq mRNA Accession(s) | XM_011524890; XM_017024739; NM_000442; XM_005276881; XM_017024738; XM_017024741; XM_005276880; XM_005276882; XM_011524889; XM_005276883; XM_017024740 |
| RefSeq Genomic Accession(s) | NG_047009; NC_000017 |
| UniProt ID(s) | P16284 |
| PharmGKB ID(s) | PA33167 |
| KEGG Gene ID(s) | hsa:5175 |
| General Description of CD31 . | |
| CD31, also known as PECAM-1, is a 130 kDa integral membrane glycoprotein found on the surface of endothelial cells, platelets and some hematopoietic cells. The antibody labels endothelial cells of arteries, arterioles, venules, veins, and non-sinusoidal capillaries invarious tissues. CD31 is the most sensitive and specific endothelial cell marker. It is useful for detection of tumors with endothelial origin. In addition, CD31 has been used to identify vascular invasion of tumors, and assessment of angiogenesis which is a prognostic marker for many types of cancer. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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