PDF DatasheetHuman, Baboon, Cynomolgus Monkey, and Rhesus Monkey Anti-CD35 / CR1 Antibody Product Attributes
Species: Human, Baboon, Cynomolgus Monkey, and Rhesus Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD35 / CR1 Antibody Clone: E11
Clone E11 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Baboon, Cynomolgus Monkey, and Rhesus Monkey CD35 / CR1 Positive Control Sample: Follicular dendritic cells (FDC) in tonsil
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Intact human monocytes
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD35 / CR1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD35 / CR1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD35 / CR1 could be seen in cells in the red pulp in spleen. We were unable to detect CD35 / CR1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD35 / CR1 General Information | |
|---|---|
| Alternate Names | |
| Heart-type fatty acid binding protein, hFABP, FABP3, mammary derived growth inhibitor | |
| Molecular Weight | |
| 210-220kDa | |
| Chromosomal Location | |
| 1q32.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CR1 |
| Entrez Gene ID | 1378 |
| Ensemble Gene ID | ENSG00000203710 |
| RefSeq Protein Accession(s) | XP_006711229, XP_011507507, NP_000564, NP_000642 |
| RefSeq mRNA Accession(s) | XM_006711166 NM_000573, NM_000651, XM_011509205 |
| RefSeq Genomic Accession(s) | NC_018912, NC_000001, NG_007481 |
| UniProt ID(s) | E9PDY4, P17927 |
| UniGene ID(s) | E9PDY4, P17927 |
| HGNC ID(s) | 2334 |
| Cosmic ID(s) | CR1 |
| KEGG Gene ID(s) | hsa:1378 |
| PharmGKB ID(s) | PA26855 |
| General Description of CD35 / CR1. | |
| Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1, shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b, C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells, follicular dendritic cell sarcoma. | |
Limitations and Warranty
enQuire Bio’s CD35 / CR1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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