PDF DatasheetHuman Anti-CD3e Antibody Product Attributes
Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-CD3e Antibody Clone: PC3/188A
Clone PC3/188A Host and Isotype: Mouse IgG1
Anti-Human CD3e Positive Control Sample: Jurkat cells. Tonsil and lymph node
Cellular Localization of Antibody Cell surface and cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-? chain
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD3e Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD3e antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3e could be seen in. We were unable to detect CD3e in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD3e General Information | |
|---|---|
| Alternate Names | |
| CD3e molecule, Cluster of Differentiation 3E, Often just referred to as CD3 | |
| Molecular Weight | |
| 20kDa | |
| Chromosomal Location | |
| 11q23.3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD3E |
| Entrez Gene ID | 916 |
| Ensemble Gene ID | ENSG00000198851 |
| RefSeq Protein Accession(s) | NP_000724 |
| RefSeq mRNA Accession(s) | NM_000733, |
| RefSeq Genomic Accession(s) | NC_018922, NG_007383, NC_000011 |
| UniProt ID(s) | P07766 |
| UniGene ID(s) | P07766 |
| HGNC ID(s) | 1674 |
| Cosmic ID(s) | CD3E |
| KEGG Gene ID(s) | hsa:916 |
| PharmGKB ID(s) | PA26216 |
| General Description of CD3e. | |
| Recognizes the ?-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. | |
Limitations and Warranty
enQuire Bio’s CD3e Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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