PDF DatasheetMouse Anti-CD4 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: RM4-5
Clone RM4-5 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD4 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD4 General Information | |
|---|---|
| Alternate Names | |
| CD4, Leu-3, CD4mut | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Cd4 |
| Entrez Gene ID | 12504 |
| Ensemble Gene ID | ENSMUSG00000023274 |
| RefSeq Protein Accession(s) | XP_006505524, XP_006505525, NP_038516 |
| RefSeq mRNA Accession(s) | NM_013488, XM_006505462, XM_006505461 |
| RefSeq Genomic Accession(s) | NC_000072 |
| UniProt ID(s) | Q3TSV7, P06332 |
| UniGene ID(s) | Q3TSV7, P06332 |
| Cosmic ID(s) | Cd4 |
| KEGG Gene ID(s) | mmu:12504 |
| General Description of CD4. | |
| The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Mouse Anti-CD4 clone GK1.5, will compete for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Mouse Anti-CD4 clone RM4-4 to cells. | |
Selected References
Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109:8670-8675. (Flow cytometry)Hofstetter AR, Ford ML, Sullivan LC, Wilson JJ, Hadley A, Brooks AG, and Lukacher AE. 2012. J. Immunol. 188:3071-3079. (in vivo depletion)Becker-Herman A, Meyer-Bahlburg A, Schwartz MA, Jackson SW, Hudkins KL, Liu C, Sather BD, Khim S, Liggitt D, Song W, Silverman GJ, Alpers CE and Rawlings DJ. 2011. J. Exp. Med. 208:2033-2042. (in vivo depletion)Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Immunofluorescence microscopy)Poitrasson-Riviere M, Bienvenu B, Le Campion A, Becourt C, Martin B, and Lucas B. 2008. J. Immunol. 180:7294-7304. (Immunohistochemistry – paraffin embedded tissue)Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry – paraffin embedded tissue)Menke J, Lucas JA, Zeller GC, Keir ME, Huang XR, Tsuboi N, Mayadas TN, Lan HY, Sharpe AH, and Kelley VR. 2007. J. Immunol. 179: 7466-7477. (Immunohistochemistry – frozen tissue)Irie J, Wu Y, Wicker LS, Rainbow D, Nalesnik MA, Hirsch R, Peterson LB, Leung PS, Cheng C, Mackay IR, Gershwin ME, and Ridgway WM. 2006. J Exp Med. 203(5):1209-19. (Immunohistochemistry – frozen tissue)Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, and Singer A. 1999. J. Exp. Med. 190: 1517-1526. (Immunoprecipitation)Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, and Datta SK. 1998. J. Exp. Med. 187:367-378. (Blocking)Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry – frozen tissue, zinc-fixed paraffin embedded tissue)
Limitations and Warranty
enQuire Bio’s Mouse Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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