PDF DatasheetHuman Anti-CD44v4 Antibody Product Attributes
CD44v4 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CD44 antibody staining is expected to be primarily localized to the golgi apparatus and plasma membrane. There is variability in either the signal strength or the localization of signal in golgi apparatus from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in CD44v4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin, glandular cells in the breast, cervix, uterine, prostate and salivary gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, melanocytes in skin, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in epidermal cells in the skin, glandular cells in the breast, cervix, uterine, prostate and salivary gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, melanocytes in skin, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina and urothelial cells in the urinary bladder. Low, but measureable presence of CD44v4 could be seen inadipocytes in mesenchymal tissue, cells in the glomeruli in kidney, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the cerebral cortex and colon, follicle cells in the ovary, glandular cells in the fallopian tube, rectum and seminal vesicle, Leydig cells in the testis, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, pneumocytes in lung and trophoblastic cells in the placenta. We were unable to detect CD44v4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD44v4 expression as measured by anti-CD44v4 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | – | +++ | ++ | ++ | +++ | +++ | + | + | +++ | +++ | + | ++ | ++ | – | ++ | – | – | ++ | – |
| CD44 Variability | ++ | + | + | ++ | ++ | ++ | + | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| CD44v4 General Information | |
|---|---|
| Alternate Names | |
| CD44, Cluster of Differentiation 44 | |
| Molecular Weight | |
| 80-95kDa | |
| Chromosomal Location | |
| 11p13 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD44 |
| Entrez Gene ID | 960 |
| Ensemble Gene ID | ENSG00000026508 |
| RefSeq Protein Accession(s) | XP_005253290, XP_011518789, XP_011518790, XP_005253288, XP_011518784, XP_011518786, NP_001001391, XP_005253297, XP_006718451, XP_011518787, XP_011518791, NP_000601, NP_001001389, NP_001189486, XP_005253289, XP_011518788, NP_001001392, NP_001189484, XP_005253292, XP_005253295, XP_005253296, XP_011518785, NP_001001390, XP_005253291, XP_006718453, XP_016874072, XP_016874074, NP_001189485, XP_016874073 |
| RefSeq mRNA Accession(s) | NM_001202556, XM_005253235, XM_006718388, NM_001001389, NM_001001391, XM_011520489, XM_011520485, XM_011520487, XM_011520488, XM_017018584, XM_017018585, NM_001202555, XM_005253231, XM_005253232, XM_005253234, XM_006718390, XM_011520483, NM_001001392, NM_001202557, XM_005253238, XM_005253239, XM_005253240, NM_001001390, XM_005253233, XM_011520482, XM_011520484, XM_011520486, XM_017018583, NM_000610 |
| RefSeq Genomic Accession(s) | NG_008937, NC_000011, NC_018922 |
| UniProt ID(s) | P16070 |
| UniGene ID(s) | P16070 |
| HGNC ID(s) | 1681 |
| Cosmic ID(s) | CD44 |
| KEGG Gene ID(s) | hsa:960 |
| PharmGKB ID(s) | PA26221 |
| General Description of CD44v4. | |
| This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies. | |
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