Anti-CD56 / NCAM1 / NKH1 Antibody Clone NCAM1/795

$ 429.00

SKU: 4684-MSM5
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

 PDF Datasheet

Human Anti-CD56 / NCAM1 / NKH1 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD56 / NCAM1 / NKH1 Antibody Clone: NCAM1/795
Clone NCAM1/795 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD56 / NCAM1 / NKH1 Positive Control Sample: Cerebellum, Pancreas, Neuroblastoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human NCAM1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD56 / NCAM1 / NKH1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NCAM1 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD56 / NCAM1 / NKH1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. Low, but measureable presence of CD56 / NCAM1 / NKH1 could be seen inglial cells in the hippocampus, exocrine glandular cells in the pancreas and smooth muscle cells in the smooth muscle. We were unable to detect CD56 / NCAM1 / NKH1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD56 / NCAM1 / NKH1 expression as measured by anti-CD56 / NCAM1 / NKH1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++
NCAM1 Variability + ++ + + + ++ + + + + + ++ + + + + + + ++ +

Limitations and Warranty

enQuire Bio’s CD56 / NCAM1 / NKH1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
Size

Tag

Buffer and Stabilizer

,

Product Type

Host

Isotype

Applications

,

Species

Mass Spec Validated?

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Anti-CD56 / NCAM1 / NKH1 Antibody Clone NCAM1/795

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-4684-MSM5-P1-anti-CD56-NCAM1-NKH1-antibody-300x225.jpeghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-4684-MSM5-P1-anti-CD56-NCAM1-NKH1-antibody-1-300x148.jpeg