PDF DatasheetMouse Anti-CD62L Antibody Product Attributes
CD62L Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD62L antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, non-germinal center cells in the lymph node, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, non-germinal center cells in the lymph node, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD62L could be seen in. We were unable to detect CD62L in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD62L expression as measured by anti-CD62L antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | + | – | + | + | + | – | + | + | – | ++ | – | + | + | + | – | – | – | + | + | + |
| SELL Variability | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | ++ | ++ | + | + | ++ | + | ++ | ++ | ++ | + | ++ | ++ |
| CD62L General Information | |
|---|---|
| Alternate Names | |
| L-Selectin, L Selectin | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Sell |
| Entrez Gene ID | 20343 |
| Ensemble Gene ID | ENSMUSG00000026581 |
| RefSeq Protein Accession(s) | XP_006496780, NP_035476, XP_006496779, XP_006496781, NP_001157531 |
| RefSeq mRNA Accession(s) | NM_001164059, XM_006496716, XM_006496717, XM_006496718, NM_011346 |
| RefSeq Genomic Accession(s) | NC_000067 |
| UniProt ID(s) | Q3TCF3, B1B507, B1B506, P18337, Q3UV83 |
| UniGene ID(s) | Q3TCF3, B1B507, B1B506, P18337, Q3UV83 |
| Cosmic ID(s) | Sell |
| KEGG Gene ID(s) | mmu:20343 |
| General Description of CD62L. | |
| The MEL-14 antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte “rolling” on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature. | |
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