PDF DatasheetHuman Anti-CD8 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD8 Antibody Clone: OKT8
Clone OKT8 Host and Isotype: Mouse IgG2a
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD8 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD8 General Information | |
|---|---|
| Alternate Names | |
| CD8A, MAL, CD8, p32, Leu2, MAL | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD8A |
| Entrez Gene ID | 925 |
| Ensemble Gene ID | ENSG00000153563 |
| RefSeq Protein Accession(s) | NP_001759, NP_001139345, NP_741969 |
| RefSeq mRNA Accession(s) | NM_001145873, NM_001768, NM_171827, NR_027353, |
| RefSeq Genomic Accession(s) | NC_000002, NG_011608, NC_018913 |
| UniProt ID(s) | P01732, Q8TAW8, Q6ZVS2 |
| UniGene ID(s) | P01732, Q8TAW8, Q6ZVS2 |
| HGNC ID(s) | 1706 |
| Cosmic ID(s) | CD8A |
| KEGG Gene ID(s) | hsa:925 |
| PharmGKB ID(s) | PA26244 |
| General Description of CD8. | |
| The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with alternative antibodies Human Anti-CD8a clone RPA-T8 or Human Anti-CD8a clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a. | |
Selected References
Jahnke M, Trowsdale J, and Kelly AP. 2012. J. Biol. Chem. 287: 28779-28789. (Flow Cytometry, Immunoprecipitation) Clement M, Ladell K, Ekeruche-Makinde J, Miles JJ, Edwards ESJ, Dolton G, Williams T, Schauenburg AJA, Cole DK, Lauder SN, Gallimore AM, Godkin AJ, Burrows SR, Price DA, Sewell AK, and Wooldridge L. 2011. J. Immunol. 187: 654-663. (in vitro activation) Bagnara D, Kaufman MS, Calissano C, Marsilio S, Patten PEM, Simone R, Chum P, Yan X-Y, Allen SL, Kolitz JE, Baskar S, Radar C, Mellstedt H, Rabbani H, Lee A, Gregersen PK, Rai KR, and Chiorazzi N. 2011. Blood. 117: 5463-5472. (in vivo activation) Teles RMB, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, and Modlin RL. 2010. 78: 4634-4643. (Immunohistochemistry – OCT embedded frozen tissue) Lai AY, Fatemi M, Dhasarathy A, Malone C, Sobol SE, Geigerman C, Jaye DL, Mav D, Shah R, Li L, and Wade PA. 2010. J. Exp. Med. 207: 1939-1950. (in vitro T cell depletion) Varghese JC and Kane KP. 2008. J. Immunol. 181: 6002-6009. (in vitro blocking) Thakral D, Dobbins J, Devine L, and Kavathas PB. 2008. J. Immunol. 180:7431-7442. (Immunoprecipitation)
Limitations and Warranty
enQuire Bio’s Human Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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