PDF DatasheetHuman, Mouse, and Rat Anti-CD86 Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD86 Antibody Clone: SPM600
Clone SPM600 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat CD86 Positive Control Sample: 293T or Jukat cells. Monocytes, dendritic cells, activated T, B, natural killer cells in lymph node or tonsil.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length CD86 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD86 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | ++ | + | ++ | ++ | + | + | ++ | ++ | ++ | ++ | + | ++ | + | – | + | ++ | ++ | ++ | ++ |
| CD86 Variability | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | ++ |
| CD86 General Information | |
|---|---|
| Alternate Names | |
| Cluster of Differentiation 86, CD86, B7-2, B7.2 | |
| Molecular Weight | |
| 70kDa | |
| Chromosomal Location | |
| 3q13.33 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD86 |
| Entrez Gene ID | 942 |
| Ensemble Gene ID | ENSG00000114013 |
| RefSeq Protein Accession(s) | NP_001193853, NP_001193854, NP_795711, NP_008820, NP_787058 |
| RefSeq mRNA Accession(s) | NM_176892, NM_001206924 NM_006889, NM_175862, NM_001206925 |
| RefSeq Genomic Accession(s) | NG_029928, NC_018914, NC_000003 |
| UniProt ID(s) | P42081, A0A0X9R4E0, A8K632 |
| UniGene ID(s) | P42081, A0A0X9R4E0, A8K632 |
| HGNC ID(s) | 1705 |
| Cosmic ID(s) | CD86 |
| KEGG Gene ID(s) | hsa:942 |
| PharmGKB ID(s) | PA26243 |
| General Description of CD86. | |
| Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein, a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes, dendritic cells, at very low density on resting B, T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28, CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin, Reed Sternberg (HRS) cells in Hodgkin’s disease. | |
Limitations and Warranty
enQuire Bio’s CD86 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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