Anti-Chromogranin A / CHGA Antibody Clone LK2H10 + PHE5

$ 429.00

SKU: 1113-MSM4
Species: Human, Monkey, Pig, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1+ IgG1
Mass Spec Validated?: Not MS Validated

 PDF Datasheet

Human, Monkey, Pig, Mouse, and Rat Anti-Chromogranin A / CHGA Antibody Product Attributes

Species: Human, Monkey, Pig, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Chromogranin A / CHGA Antibody Clone: LK2H10 + PHE5
Clone LK2H10 + PHE5 Host and Isotype: Mouse IgG1+ IgG1
Anti-Human, Monkey, Pig, Mouse, and Rat Chromogranin A / CHGA Positive Control Sample: Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma
Cellular Localization of Antibody Finely granular cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human pheochromocytoma (LK2H10 & PHE5)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Chromogranin A / CHGA Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CHGA antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Chromogranin A / CHGA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the parathyroid gland and islets of Langerhans in pancreas. More moderate antibody staining intensity was present in glandular cells in the parathyroid gland and islets of Langerhans in pancreas. Low, but measureable presence of Chromogranin A / CHGA could be seen inneuronal cells in the caudate nucleus, cerebral cortex and hippocampus, respiratory epithelial cells in the bronchus and neuronal cells in the caudate nucleus and cerebral cortex and hippocampus. We were unable to detect Chromogranin A / CHGA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Chromogranin A / CHGA expression as measured by anti-Chromogranin A / CHGA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++
CHGA Variability + + + + ++ + ++ + + + + + + + + ++ ++ + + +

Limitations and Warranty

enQuire Bio’s Chromogranin A / CHGA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-Chromogranin A / CHGA Antibody Clone LK2H10 + PHE5

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