PDF DatasheetHuman Anti-Complement 4d Antibody Product Attributes
Species: Human
Tested Applications: ELISA, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: ELISA (For coating use Ab at 1-2ug of antibody/ml, order Ab without BSA), Immunofluorescence (1:50-1:100), Immunohistochemistry (IHC) (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)
Clonality: Monoclonal
Anti-Complement 4d Antibody Clone: SPM545
Clone SPM545 Host and Isotype: Mouse IgG1 kappa
Anti-Human Complement 4d Positive Control Sample: Rejected Renal Transplant Tissue
Cellular Localization of Antibody Intracytoplasmic vacuoles of endothelial cells, Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Complement 4d protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Complement 4d Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Complement 4d antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Complement 4d could be seen in. We were unable to detect Complement 4d in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| Complement 4d General Information | |
|---|---|
| Alternate Names | |
| Complement C4-A, C4A, Often misspelled as Compliment C4 | |
| Molecular Weight | |
| 192kDa (predicted) | |
| Chromosomal Location | |
| 6p21.3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | C4A |
| Entrez Gene ID | 720, 721 |
| Ensemble Gene ID | ENSG00000227746, ENSG00000206340, ENSG00000244731, ENSG00000244207, ENSG00000224389, ENSG00000224639, ENSG00000228454, ENSG00000228267, ENSG00000236625 |
| RefSeq Protein Accession(s) | NP_001239133, NP_009224, XP_016885778, NP_001002029 |
| RefSeq mRNA Accession(s) | XM_017030289, NM_007293 NM_001252204NM_001002029 |
| RefSeq Genomic Accession(s) | NC_000006, NG_167248, NG_011638, NG_167247, NG_167245, NC_018917NG_011639, NC_018917, NG_167245, NG_113891, NG_167249, NC_000006 |
| UniProt ID(s) | Q13906, Q14835, Q9NPK5, Q9UIP5, P0C0L4, P01028, P78445, Q13160, Q14033, P0C0L4, P0C0L5 |
| UniGene ID(s) | Q13906, Q14835, Q9NPK5, Q9UIP5, P0C0L4, P01028, P78445, Q13160, Q14033, P0C0L4, P0C0L5 |
| HGNC ID(s) | 1323, 1324 |
| Cosmic ID(s) | C4A, C4B |
| KEGG Gene ID(s) | hsa:720, hsa:721 |
| PharmGKB ID(s) | PA25904, PA25903, PA25904, PA25903 |
| General Description of Complement 4d. | |
| This MAb is specific to Complement 4d (C4d), it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation, degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces, extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas, lung allografts. As an established marker of antibody-mediated acute renal allograft rejection, its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection,, borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt, aggressive anti-rejection treatment. | |
Limitations and Warranty
enQuire Bio’s Complement 4d Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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