PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Cyclooxygenase-2 Information for Pathologists
Summary:
Not related to Cytochrome c oxidase (COX). Cyclooxygenases 1 (COX1) and 2 (COX2), also known as prostaglandin H synthase, catalyze formation of prostaglandin from arachidonic acid. COX1 is constitutive form of enzyme on 9q; COX2 is inducible isoform on 1q, has 61% sequence homology with COX1. Regulated by mitogens, tumor promoters, cytokines, serum, free fatty acids, NSAIDs, selective COX2 inhibitors. Increased expression associated with poor clinical outcome in stage I/II non-small cell lung carcinoma (Arch Pathol Lab Med 2005;129:1113), possibly poorer outcome in follicular thyroid carcinoma (Arch Pathol Lab Med 2005;129:736).
Common Uses By Pathologists:
Endothelial cells (cytoplasmic) as well as breast, colorectal, esophageal, liver, lung, ovary, pancreas, prostate, skin, stomach tumors stain positive for COX2.
| Cyclooxygenase-2 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 69 kDa | |
| Chromosomal Location | |
| q31.1 [chr: 1] [chr_start: 186671791] [chr_end: 186680423] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PTGS2 |
| Entrez Gene ID | 5743 |
| RefSeq Protein Accession(s) | NP_000954 |
| RefSeq mRNA Accession(s) | NM_000963; |
| RefSeq Genomic Accession(s) | NG_028206; NC_000001 |
| UniProt ID(s) | P35354 |
| PharmGKB ID(s) | PA293 |
| KEGG Gene ID(s) | hsa:5743 |
| General Description of Cyclooxygenase-2 . | |
| This antibody reacts with a 70 kDa protein. COX-2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters and cytokines that induce its expression. COX-2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX-1 levels remained unchanged. | |
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