PDF DatasheetHuman Anti-Cytokeratin 10/13 Antibody Product Attributes
Species: Human.
Predicted Cross-Reactivity: Feline.
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.25-0.5ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Cytokeratin 10/13 Antibody Clone: DE-K13
Clone DE-K13 Host and Isotype: Mouse IgG2a kappa
Anti-Human Cytokeratin 10/13 Positive Control Sample: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 1.0mg/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 10/13 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Cytokeratin 10/13 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin and keratinocytes in skin. More moderate antibody staining intensity was present in epidermal cells in the skin and keratinocytes in skin. Low, but measureable presence of Cytokeratin 10/13 could be seen in. We were unable to detect Cytokeratin 10/13 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| Cytokeratin 10/13 General Information | |
|---|---|
| Alternate Names | |
| Keratin, type I cytoskeletal 10, cytokeratin-10, CK-10, keratin-10, K10, KRT10 | |
| Molecular Weight | |
| 56.5kDa (CK10) & 53kDa (CK13) | |
| Chromosomal Location | |
| 17q21.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT10 |
| Entrez Gene ID | 3858 |
| Ensemble Gene ID | ENSG00000186395 |
| RefSeq Protein Accession(s) | XP_005257400, NP_000412 |
| RefSeq mRNA Accession(s) | NM_000421, XM_005257343 |
| RefSeq Genomic Accession(s) | NC_018928, NG_033147, NG_008405, NC_000017 |
| UniProt ID(s) | P13645 |
| UniGene ID(s) | P13645 |
| HGNC ID(s) | 6413 |
| Cosmic ID(s) | KRT10 |
| KEGG Gene ID(s) | hsa:3858 |
| PharmGKB ID(s) | PA30200 |
| General Description of Cytokeratin 10/13. | |
| This antibody recognizes cytokeratin 10 (56.5kDa), cytokeratin 13 (53kDa) in Western Blot. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying, non-cornifying stratified epithelia, more differentiated cells of squamous carcinomas. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin 10/13 Anti-Human, Cat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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