PDF DatasheetHuman, Pig, Mouse, Rat, Hamster, Cow, Canine, Sheep, ferret, Chicken (-), and Xenopus (-) Anti-Cytokeratin 5/8 Antibody Product Attributes
Species: Human, Pig, Mouse, Rat, Hamster, Cow, Canine, Sheep, ferret, Chicken (-), and Xenopus (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 5/8 Antibody Clone: SPM268
Clone SPM268 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Pig, Mouse, Rat, Hamster, Cow, Canine, Sheep, ferret, Chicken (-), and Xenopus (-) Cytokeratin 5/8 Positive Control Sample: HepG2 or A431 cells. Skin or Squamous cell carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS, pH 7.4 with 0.05% BSA & 0.05% sodium azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Cytoskeletal preparation from HeLa cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 5/8 Previously Observed Antibody Staining Patterns
| Cytokeratin 5/8 General Information | |
|---|---|
| Alternate Names | |
| CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8, anti-CK5 antibody, anti-DDD antibody, anti-EBS2 antibody, anti-Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types antibody, anti-K5 antibody, anti-Keratin complex 2 antibody, anti-basic antibody, anti-Gene 5 antibody, anti-Keratin Type II Cytoskeletal 5 antibody, anti-Keratin-5 antibody, anti-KRT5 antibody, anti-KRT5A antibody, anti-Type II Cytoskeletal 5 antibody, anti-Type-II Keratin Kb5. CK8 antibody, anti-CARD2 antibody, anti-CYK8 antibody, anti-CYKER antibody, anti-Cytokeratin Endo A antibody, anti-DreK8 antibody, anti-EndoA antibody, anti-K2C8 antibody, anti-K8 antibody, anti-Keratin 8 antibody, anti-Krt 2.8 antibody, anti-KRT8 antibody, anti-Type-II Keratin Kb8 antibody | |
| Molecular Weight | |
| 58kDa (CK5); 52.5kDa (CK8) | |
| Chromosomal Location | |
| 12q13.13 (CK5) & 17q21.2 (CK8) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT5 & KRT8 |
| Entrez Gene ID | 3852, 3856 |
| Ensemble Gene ID | ENSG00000186081, ENSG00000170421 |
| RefSeq Protein Accession(s) | NP_000415, NP_001243211, NP_002264, NP_001243222 |
| RefSeq mRNA Accession(s) | NM_000424, NR_045962 NM_001256282, NM_001256293, NM_002273 |
| RefSeq Genomic Accession(s) | NG_008297, NC_000012, NC_018923, NC_000012, NC_018923, NG_008402 |
| UniProt ID(s) | P13647, P05787, Q7L4M3 |
| UniGene ID(s) | P13647, P05787, Q7L4M3 |
| HGNC ID(s) | 6442, 6446 |
| Cosmic ID(s) | KRT5, KRT8 |
| KEGG Gene ID(s) | hsa:3852, hsa:3856 |
| PharmGKB ID(s) | PA30230, PA30234 |
| General Description of Cytokeratin 5/8. | |
| It reacts with keratin 5 (58kDa), keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia, their carcinomas. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin 5/8 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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