PDF DatasheetHuman Anti-Cytokeratin 8/18 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 8/18 Antibody Clone: SPM141
Clone SPM141 Host and Isotype: Mouse IgG1 kappa
Anti-Human Cytokeratin 8/18 Positive Control Sample: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Keratin preparation from human breast carcinoma MCF-7 cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 8/18 Previously Observed Antibody Staining Patterns
| Cytokeratin 8/18 General Information | |
|---|---|
| Alternate Names | |
| CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18, anti-CARD2 antibody, anti-CK8 antibody, anti-CYK8 antibody, anti-CYKER antibody, anti-Cytokeratin Endo A antibody, anti-DreK8 antibody, anti-EndoA antibody, anti-K2C8 antibody, anti-K8 antibody, anti-Keratin 8 antibody, anti-Krt 2.8 antibody, anti-KRT8 antibody, anti-Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein antibody, anti-CK18 antibody, anti-CYK18 Cytokeratin Endo B antibody, anti-K18 antibody, anti-Keratin-18 antibody, anti-Kerd antibody, anti-KRT18 antibody | |
| Molecular Weight | |
| 52.5kDa (CK8); 45kDa (CK18) | |
| Chromosomal Location | |
| 17q21.2 (CK8); 12q13.13 (CK18) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT8 & KRT18 |
| Entrez Gene ID | 3856, 3875 |
| Ensemble Gene ID | ENSG00000170421, ENSG00000111057 |
| RefSeq Protein Accession(s) | NP_001243211, NP_002264, NP_001243222, NP_954657, NP_000215 |
| RefSeq mRNA Accession(s) | NR_045962 NM_001256282, NM_001256293, NM_002273, NM_199187, NM_000224 |
| RefSeq Genomic Accession(s) | NC_000012, NC_018923, NG_008402, NC_000012, NG_008351, NG_008402, NC_018923 |
| UniProt ID(s) | P05787, Q7L4M3, P05783, A0A024RAY2 |
| UniGene ID(s) | P05787, Q7L4M3, P05783, A0A024RAY2 |
| HGNC ID(s) | 6446, 6430 |
| Cosmic ID(s) | KRT8, KRT18 |
| KEGG Gene ID(s) | hsa:3856, hsa:3875 |
| PharmGKB ID(s) | PA30234, PA30217 |
| General Description of Cytokeratin 8/18. | |
| Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins, exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract,, urogenital tract including transitional epithelium. All adenocarcinomas, most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin 8/18 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
![Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 8/18 MS-QAVA™ monoclonal antibody [Clone: B22.1/B23.1] (solid-line), reveals that less than 4.1% of signal is attributable to non-specific binding of anti-Cytokeratin 8/18 [Clone B22.1/B23.1] to targets other than KRT8 & KRT18 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-MSM20-1221-P1-anti-Cytokeratin-818-antibody-150x150.png)
![Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 8/18 MS-QAVA™ monoclonal antibody [Clone: C-51] (solid-line), reveals that less than 3.8% of signal is attributable to non-specific binding of anti-Cytokeratin 8/18 [Clone C-51] to targets other than KRT8 & KRT18 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-MSM19-34-P1-anti-Cytokeratin-818-antibody-150x150.png)
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