Human, Mouse, and Rat Anti-Cytokeratin Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: SPM591
Clone SPM591 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat Cytokeratin Positive Control Sample: PC12 cells, Skin, Prostate carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Solubilized keratin extract from human stratum corneum
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin Previously Observed Antibody Staining Patterns
Cytokeratin General Information | |
---|---|
Molecular Weight | |
67kDa (CK1); 58kDa (CK5); 56.5kDa (CK10); 50kDa (CK14) | |
Chromosomal Location | |
Multiple | |
Curated Database and Bioinformatic Data | |
Gene Symbol | Multiple |
Entrez Gene ID | 3848, 3852, 3858, 3861 |
Ensemble Gene ID | ENSG00000167768, ENSG00000186081, ENSG00000186395, ENSG00000186847 |
RefSeq Protein Accession(s) | NP_006112, NP_000415, XP_005257400, NP_000412, NP_000517 |
RefSeq mRNA Accession(s) | NM_006121, NM_000424, NM_000421, XM_005257343NM_000526 |
RefSeq Genomic Accession(s) | NG_008364, NC_000012, NC_018923, NG_008297, NC_000012, NC_018923, NC_018928, NG_033147, NG_008405, NC_000017NG_008624, NC_000017, NC_018928 |
UniProt ID(s) | P04264, P13647, P13645, P02533 |
UniGene ID(s) | P04264, P13647, P13645, P02533 |
HGNC ID(s) | 6412, 6442, 6413, 6416 |
Cosmic ID(s) | KRT1, KRT5, KRT10, KRT14 |
KEGG Gene ID(s) | hsa:3848, hsa:3852, hsa:3858, hsa:3861 |
PharmGKB ID(s) | PA30199, PA30230, PA30200, PA30203 |
General Description of Cytokeratin. | |
This antibody recognizes CK1, CK5, CK10, CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells, basal cells in the prostate gland, bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors,, neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells, has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. |
Limitations and Warranty
enQuire Bio’s Cytokeratin, Basic Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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