PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
DOG 1 Information for Pathologists
Summary:
Discovered On GIST 1. Chloride channel protein. Interpretation Membranous and cytoplasmic staining. Uses by pathologists
Common Uses By Pathologists:
Sensitive and specific marker for GIST (Gut Liver 2011;5:171). May be more sensitive and specific for GIST than CD117 / KIT in cell blocks (Cancer Cytopathol 2011;119:202, Am J Clin Pathol 2011;135:448). Sensitive for CD117 negative GIST tumor (non-GI tumors are usually DOG1+, Am J Surg Pathol 2011;35:1287, Mod Pathol 2011;24:866). Negative staining suggests tumor is not GIST (Cancer Control 2011;18:152). Distinguish gastric plexiform fibromyxoma (alpha smooth muscle actin+, KIT-, DOG1-, CD34-, no KIT / PDGFRA mutations) from GIST (opposite, Am J Surg Pathol 2009;33:1624).
| DOG 1 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 114.1 kDa | |
| Chromosomal Location | |
| q13.3 [chr: 11] [chr_start: 70078302] [chr_end: 70189528] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ANO1 |
| Entrez Gene ID | 55107 |
| RefSeq Protein Accession(s) | NP_060513; XP_011543433; XP_006718668 |
| RefSeq mRNA Accession(s) | XM_011545121; XM_011545128; NR_030691; XM_011545124; XM_011545131; XM_006718602; XM_011545123; XM_011545126; XM_011545127; XM_006718604; XM_011545129; XM_017017957; NM_018043; XM_006718605; XM_011545125; XM_017017956 |
| RefSeq Genomic Accession(s) | NC_000011 |
| UniProt ID(s) | Q5XXA6 |
| PharmGKB ID(s) | PA164715378 |
| KEGG Gene ID(s) | hsa:55107 |
| General Description of DOG 1 . | |
| DOG1 is expressed ubiquitously in gastrointestinal stromal tumors irrespective of c-kit or PDGFR alpha mutation status. Reactivity for DOG1 may aid in the diagnosis of gastrointestinal stromal tumors, including PDGFRA mutants that fail to express c-kit antigen, and lead to appropriate treatment with imatinib mesylate, an inhibitor of the c-kit tyrosine kinase. | |
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