PDF DatasheetDISCONTINUED: Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:100 – 1:200 |
| Pretreatment | EDTA Buffer pH8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Epithelial Antigen (EpCAM) Antibody Clone: EP155
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Colon for normal tissue and colorectal cancer for tumor tissue
Cellular Localization of Antibody EP155 Staining: Membrane/Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to amino acid residues in human Epithelial Antigen (Ep-CAM) protein was used as an immunogen
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Epithelial Antigen (EpCAM) Information for Pathologists
| Epithelial Antigen (EpCAM) General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 34.9 kDa | |
| Chromosomal Location | |
| p21 [chr: 2] [chr_start: 47345158] [chr_end: 47387601] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EPCAM |
| Entrez Gene ID | 4072 |
| RefSeq Protein Accession(s) | NP_002345 |
| RefSeq mRNA Accession(s) | NM_002354; |
| RefSeq Genomic Accession(s) | NG_012352; NC_000002 |
| UniProt ID(s) | P16422 |
| PharmGKB ID(s) | PA35493 |
| KEGG Gene ID(s) | hsa:4072 |
| Associated Diseases (KEGG IDs) | Diarrhea 5, with tufting enteropathy, congenital (DIAR5) [MIM:613217]: An intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum. {ECO:0000269|PubMed:18572020}. The disease is caused by mutations affecting the gene represented in this entry.; Hereditary non-polyposis colorectal cancer 8 (HNPCC8) [MIM:613244]: An autosomal dominant disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term ‘suspected HNPCC’ or ‘incomplete HNPCC’ can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. {ECO:0000269|PubMed:19098912}. The disease is caused by mutations affecting the gene represented in this entry. HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. |
| General Description of Epithelial Antigen (EpCAM) . | |
| Ep-CAM is a highly conserved typeI transmembrane glycoprotein and is expressed on most normal and malignant epithelial cells. Ep-CAM is also known as epithelial cell adhesion molecule. It is detected at the membrane/cytoplasm of epithelial tissues. Ep-CAM is over expressed by the majority of human epithelial carcinomas except hepatocellular carcinoma. An antibody to EP-CAM is useful in identification of epithelial tumors and to differentiate primary hepatocellular carcinoma from liver metastasis | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
There are no reviews yet.