PDF DatasheetHuman, Monkey, Mouse, Rat, Pig, Horse, and Sheep Anti-ER-beta1 Antibody Product Attributes
Species: Human, Monkey, Mouse, Rat, Pig, Horse, and Sheep
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-ER-beta1 Antibody Clone: ERb455
Clone ERb455 Host and Isotype: Mouse IgG2a
Anti-Human, Monkey, Mouse, Rat, Pig, Horse, and Sheep ER-beta1 Positive Control Sample: MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma
Cellular Localization of Antibody Predominantly nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: C-terminus fragment of recombinant human estrogen receptor beta protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
ER-beta1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-ESR2 antibody staining is expected to be primarily localized to the nucleoplasm.
| ER-beta1 General Information | |
|---|---|
| Alternate Names | |
| Estrogen receptor beta, ER-?, ERbeta, ER beta, NR3A2, nuclear receptor subfamily 3, group A, member 2, ESR2 | |
| Molecular Weight | |
| 53-59kDa | |
| Chromosomal Location | |
| 14q23.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ESR2 |
| Entrez Gene ID | 2100 |
| Ensemble Gene ID | ENSG00000140009 |
| RefSeq Protein Accession(s) | XP_016876568, XP_016876571, NP_001035365, NP_001201831, NP_001258806, XP_016876569, XP_016876573, XP_016876572, NP_001258805, NP_001278641, NP_001278652, XP_016876570, NP_001428 |
| RefSeq mRNA Accession(s) | NM_001214902, NM_001271876, NM_001437, NR_073497, XM_017021080, XM_017021083, XM_017021082 NM_001291723, NM_001040276, XR_001750187, NM_001291712, NM_001040275, NM_001271877, NM_001214903, XM_017021079, XM_017021081, XM_017021084, NR_073496 |
| RefSeq Genomic Accession(s) | NC_000014, NG_011535, NC_018925 |
| UniProt ID(s) | Q7LCB3, F1D8N3, Q92731, Q0PTK2 |
| UniGene ID(s) | Q7LCB3, F1D8N3, Q92731, Q0PTK2 |
| HGNC ID(s) | 3468 |
| Cosmic ID(s) | ESR2 |
| KEGG Gene ID(s) | hsa:2100 |
| PharmGKB ID(s) | PA27886 |
| General Description of ER-beta1. | |
| Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha, ER-beta, contain DNA binding, ligand binding domains, are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane, can be rapidly activated by exposure of cells to estrogen. ER-alpha, ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation, the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha, ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. | |
Limitations and Warranty
enQuire Bio’s ER-beta1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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