PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | Only Available as Predilute |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
ERG Information for Pathologists
Summary:
Transcription factor highly specific and 50% sensitive for prostatic adenocarcinoma. Detect via FISH or immunohistochemistry. Terminology V-ets erythroblastosis virus E26 oncogen homologue. ETS family transcription factor present at 22q12.
Notable Clinical Features:
TMPRSS2-ERG fusion may be an early event in prostate cancer (Arch Pathol Lab Med 2009;133:1033), although marked intrafocal heterogeneity exists (Mod Pathol 2013;26:106). ERG does not appear to have prognostic value in prostate adenocarcinoma after radical prostatectomy (Mod Pathol 2012;25:471) or in high grade PIN (BJU Int 2012;110:E751). ERG rearrangement is more common in peripheral zone prostatic adenocarcinomas, but also present in 12% of transition zone cancers (Mod Pathol 2010;23:1499). Similar TMPRSS2-ERG gene fusion status between primary and metastatic prostate cancers (Hum Pathol 2012;43:644). Quantification of T2:ERG in postdigital rectal examination urine is specific for prostate cancer (Am J Clin Pathol 2012;138:685).
Common Uses By Pathologists:
TMPRSS2-ERG fusion is one of most common translocations for any malignancy. 50% sensitive and highly specific for prostate adenocarcinoma, usually by FISH (Am J Surg Pathol 2007;31:882). Lower frequency (28%) in patients from Japan (Mod Pathol 2010;23:1492) and Korea (Korean J Pathol 2012;46:423). ERG immunohistochemistry (nuclear stain) accurately assesses presence of TMPRSS2-ERG fusion protein. Simpler and less costly than FISH (Am J Surg Pathol 2011;35:1014).
| ERG General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 54.6 kDa | |
| Chromosomal Location | |
| q22.2 [chr: 21] [chr_start: 38380027] [chr_end: 38661780] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ERG |
| Entrez Gene ID | 2078 |
| RefSeq Protein Accession(s) | NP_001129627; NP_001230358; NP_891548; NP_004440; NP_001230361; NP_001129626; NP_001230357; NP_001278320 |
| RefSeq mRNA Accession(s) | NM_001243428; NM_001243429; NM_001136154; NM_001136155; NM_001243432; NM_001331025; NM_001243433; NR_111949; NM_004449; NM_182918; NM_001291391 |
| RefSeq Genomic Accession(s) | NG_029732; NC_000021 |
| UniProt ID(s) | P11308 |
| PharmGKB ID(s) | PA27858 |
| KEGG Gene ID(s) | hsa:2078 |
| Associated Diseases (KEGG IDs) | Ewing sarcoma (ES) [MIM:612219]: A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. {ECO:0000269|PubMed:8076344}. The gene represented in this entry is involved in disease pathogenesis. A chromosomal aberration involving ERG has been found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1. {ECO:0000269|PubMed:8076344}.; Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS (PubMed:8187069). Translocation t(X;21)(q25-26;q22) with ELF4 (PubMed:16303180). {ECO:0000269|PubMed:16303180, ECO:0000269|PubMed:8187069}. |
| General Description of ERG . | |
| ERG, the ETS related gene, belongs to the ETS family that plays important roles in cell development, differentiation, proliferation, apoptosis and tissue remodeling. The ERG antibody identifies endothelial cells, lymphocytes and prostate cancer cells. | |
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