PDF DatasheetMouse Anti-F4/80 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-F4/80 Antibody Clone: BM8.1
Clone BM8.1 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
F4/80 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of F4/80 expression as measured by anti-F4/80 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| ADGRE1 Variability | + | ++ | + | + | ++ | ++ | + | + | + | + | + | ++ | + | ++ | + | + | + | + | + | + |
| F4/80 General Information | |
|---|---|
| Alternate Names | |
| EGF-like module-containing mucin-like hormone receptor-like 1, EFR1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Adgre1 |
| Entrez Gene ID | 13733 |
| Ensemble Gene ID | ENSMUSG00000004730 |
| RefSeq Protein Accession(s) | NP_034260, XP_006523665, XP_006523664 |
| RefSeq mRNA Accession(s) | XM_006523601, NM_010130, XM_006523602 |
| RefSeq Genomic Accession(s) | NC_000083 |
| UniProt ID(s) | Q61549, Q3U9R0 |
| UniGene ID(s) | Q61549, Q3U9R0 |
| Cosmic ID(s) | Adgre1 |
| KEGG Gene ID(s) | mmu:13733 |
| General Description of F4/80. | |
| The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry. | |
Selected References
Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry)Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157. Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055. Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry – paraffin-embedded tissue)Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry – frozen tissue)
Limitations and Warranty
enQuire Bio’s Mouse Anti-F4/80 Antigen Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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