PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | Enzymatic Antigen Retrieval (eg. Histozyme) |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Factor VIII Related /Von Willebrand Factor Information for Pathologists
Summary:
Large glycoprotein involved in platelet aggression and adhesion to subendothelial matrix. Somewhat sensitive (50-75%) for vascular tumors; CD31, CD34 or perhaps FLI1 are more sensitive. Interpretation Cytoplasmic stain. Endothelium should be a positive internal control.
| Factor VIII Related /Von Willebrand Factor General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 298.4 kDa | |
| Chromosomal Location | |
| p13.31 [chr: 12] [chr_start: 5948874] [chr_end: 6124770] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | VWF |
| Entrez Gene ID | 7450 |
| RefSeq Protein Accession(s) | NP_000543; |
| RefSeq mRNA Accession(s) | NM_000552; |
| RefSeq Genomic Accession(s) | NC_000012; NG_009072 |
| UniProt ID(s) | L8E853 |
| KEGG Gene ID(s) | hsa:7450 |
| General Description of Factor VIII Related /Von Willebrand Factor . | |
| This antibody reacts with Von Willebrand factor in endothelial cells. It also reacts with megakaryocytes in human bone marrow. | |
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