PDF DatasheetHuman, Pig, Mouse, and Rat Anti-Fibronectin Antibody Product Attributes
Fibronectin Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Fibronectin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the tubules in kidney. More moderate antibody staining intensity was present in cells in the tubules in kidney. Low, but measureable presence of Fibronectin could be seen inadipocytes in breast, cells in the endometrial stroma in endometrium, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, cells in the white pulp in spleen, glandular cells in the appendix, breast, epididymis, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, hematopoietic cells in the bone marrow, hepatocytes in liver, macrophages in lung, neuronal cells in the hippocampus, peripheral nerve in mesenchymal tissue, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the esophagus and oral mucosa, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. We were unable to detect Fibronectin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Fibronectin expression as measured by anti-Fibronectin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | + | – | – | – | ++ | + | – | – | – | ++ | – | – | – | – | + | ++ | + |
| FN1 Variability | ++ | ++ | ++ | +++ | ++ | + | ++ | +++ | ++ | + | ++ | ++ | +++ | + | + | + | ++ | ++ | ++ | +++ |
| Fibronectin General Information | |
|---|---|
| Alternate Names | |
| Fibronectin, FN1 | |
| Molecular Weight | |
| 220kDa (monomer); 440kDa (dimer) | |
| Chromosomal Location | |
| 2q35 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FN1 |
| Entrez Gene ID | 2335 |
| Ensemble Gene ID | ENSG00000115414 |
| RefSeq Protein Accession(s) | XP_005246460, XP_005246464, XP_005246473, NP_997641, XP_005246461, XP_005246467, XP_005246469, XP_016859183, NP_001293060, NP_997639, NP_997647, XP_005246454, XP_005246466, XP_005246471, XP_016859182, NP_001293059, XP_005246458, XP_016859181, NP_001293058, XP_005246465, XP_005246468, NP_473375, XP_005246455, XP_005246459, NP_002017, NP_997643, XP_005246462, XP_005246456, XP_005246463, XP_016859184, NP_001293061 |
| RefSeq mRNA Accession(s) | XM_005246416, XM_017003692 NM_212476, XM_005246407, XM_005246410, XM_005246414, XM_017003694, NM_001306129, NM_001306130, NM_212474, XM_005246402, XM_005246408, XM_005246409, XM_005246399, NM_001306131, NM_054034, XM_005246403, XM_005246405, XM_005246406, XM_017003695, NM_002026, XM_005246398, XM_005246401, XM_005246404, XM_005246412, XM_017003693, NM_001306132, XM_005246397, XM_005246411, NM_212478, NM_212482, NM_212475 |
| RefSeq Genomic Accession(s) | NC_000002, NG_012196, NC_018913 |
| UniProt ID(s) | Q6N084, Q9UQS6, B7ZLE5, Q6MZF4, Q6MZM7, P02751 |
| UniGene ID(s) | Q6N084, Q9UQS6, B7ZLE5, Q6MZF4, Q6MZM7, P02751 |
| HGNC ID(s) | 3778 |
| Cosmic ID(s) | FN1 |
| KEGG Gene ID(s) | hsa:2335 |
| PharmGKB ID(s) | PA28194 |
| General Description of Fibronectin. | |
| Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids, in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells,, it has been shown to be involved in various functions including cell adhesion, cell motility, wound healing. Alternative splicing, glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin, Fibronectin splice variants in Dupuytrens disease. | |
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