PDF DatasheetHuman Anti-FLI1 Antibody Product Attributes
Species: Human
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-FLI1 Antibody Clone: FLI1/1312
Clone FLI1/1312 Host and Isotype: Mouse IgG
Anti-Human FLI1 Positive Control Sample: THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (aa 107-261) of human FLI1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
FLI1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-FLI1 antibody staining is expected to be primarily localized to the nuclear bodies and nucleus. There is variability in either the signal strength or the localization of signal in nuclear bodies from cell to cell.
| FLI1 General Information | |
|---|---|
| Alternate Names | |
| Friend leukemia integration 1 transcription factor, FLI1, transcription factor ERGB, | |
| Molecular Weight | |
| 51kDa | |
| Chromosomal Location | |
| 11q24.1-q24.3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FLI1 |
| Entrez Gene ID | 2313 |
| Ensemble Gene ID | ENSG00000151702 |
| RefSeq Protein Accession(s) | NP_001257941, XP_011541004, XP_016872895, XP_016872894, NP_001257939, NP_002008, XP_011541003, NP_001161153 |
| RefSeq mRNA Accession(s) | XM_011542701, XM_017017406 NM_001271012, NM_001271010, NM_002017, XM_011542702, XM_017017405, NM_001167681 |
| RefSeq Genomic Accession(s) | NC_000011, NC_018922, NG_032912 |
| UniProt ID(s) | A0A024R3K4, A0A024R3M5, A0A024R3N6, Q01543 |
| UniGene ID(s) | A0A024R3K4, A0A024R3M5, A0A024R3N6, Q01543 |
| HGNC ID(s) | 3749 |
| Cosmic ID(s) | FLI1 |
| KEGG Gene ID(s) | hsa:2313 |
| PharmGKB ID(s) | PA28170 |
| General Description of FLI1. | |
| Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII. | |
Limitations and Warranty
enQuire Bio’s FLI1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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