PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Galectin-3 Information for Pathologists
Summary:
Member of carbohydrate-binding protein family known as lectins. One of 14 galectins, which function as cell receptors for N-acetyl-lactosamine moieties present on most extracellular matrix components. Also member of the beta-galactoside-binding protein family that plays an important role in cell – cell adhesion, cell – matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. Uses by pathologists In one study, Gal-3+ with Ki67 > 6% was associated with parathyroid carcinomas vs. adenomas (Hum Pathol 2005;36:908).
Common Uses By Pathologists:
In one study, Gal-3+ with Ki67 > 6% was associated with parathyroid carcinomas vs. adenomas (Hum Pathol 2005;36:908). Positive staining – normal Endothelial cells, peripheral nerve, folliculostellate cells of adenohypophysis. Positive staining – disease Tumors of thyroid, head and neck, liver, colon, prolactinomas; parathyroid carcinoma; rarely in reactive of hyperplastic parathyroid lesions.
| Galectin-3 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 26.2 kDa | |
| Chromosomal Location | |
| q22.3 [chr: 14] [chr_start: 55124110] [chr_end: 55145423] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LGALS3 |
| Entrez Gene ID | 3958 |
| RefSeq Protein Accession(s) | NP_002297 |
| RefSeq mRNA Accession(s) | NM_001357678; NM_002306; NR_003225; NM_001177388 |
| RefSeq Genomic Accession(s) | NG_017089; NC_000014; |
| UniProt ID(s) | P17931 |
| PharmGKB ID(s) | PA30340 |
| KEGG Gene ID(s) | hsa:3958 |
| General Description of Galectin-3 . | |
| Galectin-3 is a member of the beta-galactoside-binding protein family that plays an important role in cell adhesion, cell matrix interactions, macrophage activation, angiogenesis, metastasis, and apoptosis. | |
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