PDF DatasheetHuman Anti-GARP Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-GARP Antibody Clone: GARP5
Clone GARP5 Host and Isotype: Mouse IgG1
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
GARP Previously Observed Antibody Staining Patterns
| GARP General Information | |
|---|---|
| Alternate Names | |
| LRRC32, Latent TGF beta receptor | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LRRC32 |
| Entrez Gene ID | 2615 |
| Ensemble Gene ID | ENSG00000137507 |
| RefSeq Protein Accession(s) | NP_001122394, XP_011543237, XP_005273960, NP_005503, XP_005273959, XP_016873016 |
| RefSeq mRNA Accession(s) | XM_005273903, XM_005273902, XM_011544935, NM_001128922, NM_005512, XM_017017527 |
| RefSeq Genomic Accession(s) | NC_000011, NC_018922 |
| UniProt ID(s) | Q14392, A0A024R5J7 |
| UniGene ID(s) | Q14392, A0A024R5J7 |
| HGNC ID(s) | 4161 |
| Cosmic ID(s) | LRRC32 |
| KEGG Gene ID(s) | hsa:2615 |
| PharmGKB ID(s) | PA28574 |
| General Description of GARP. | |
| The GARP5 antibody reacts with human GARP, also known as LRRC32 or Garpin, an 80 kDa transmembrane protein which acts as a receptor for the latent form of TGF-beta 1 (pro-TGF-beta), preventing its secretion. Specifically, GARP is reported to associate with the pro-domain of TGF-beta 1, known as latency-associated peptide (LAP), which must be cleaved to release the biologically active cytokine. The putative role of GARP in sequestering the TGF-beta is important for regulating its activity, as TGF-beta signaling is involved in development and function of Th17, Treg and many other immune cell types. GARP expression has been shown on platelets and at high levels on Foxp3+ Treg cells, where it is proposed to be a phenotypic identifier for activated human Treg cells.The GARP5 antibody may be used for analysis of human GARP by flow cytometry, immunoprecipitation and Western Blot. | |
Selected References
Wang I, Zhu J, Dong X, Shi M, Lu C, and Springer TA. 2012. Mol. Biol. Cell. 23: 1129 – 1139. (Flow cytometry, Immunoprecipitation, Western blot).
Limitations and Warranty
enQuire Bio’s Human Anti-GARP Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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