PDF DatasheetHuman, Mouse, Rat, Cow, Pig, Rabbit, and Chicken Anti-GFAP Antibody Product Attributes
Species: Human, Mouse, Rat, Cow, Pig, Rabbit, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-GFAP Antibody Clone: GA-5 + ASTRO/789
Clone GA-5 + ASTRO/789 Host and Isotype: Mouse IgG
Anti-Human, Mouse, Rat, Cow, Pig, Rabbit, and Chicken GFAP Positive Control Sample: Brain or Astrocytoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: GFAP isolated from pig spinal cord (GA-5); Recombinant GFAP protein (ASTRO/789)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
GFAP Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-GFAP antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in GFAP antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of GFAP could be seen in. We were unable to detect GFAP in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| GFAP General Information | |
|---|---|
| Alternate Names | |
| GFAP, Glial fibrillary acidic protein | |
| Molecular Weight | |
| ~50kDa | |
| Chromosomal Location | |
| 17q21.31 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GFAP |
| Entrez Gene ID | 2670 |
| Ensemble Gene ID | ENSG00000131095 |
| RefSeq Protein Accession(s) | NP_001124491, XP_016879940, NP_001229305, NP_002046 |
| RefSeq mRNA Accession(s) | XM_017024451, NM_002055 NM_001242376, NM_001131019 |
| RefSeq Genomic Accession(s) | NC_018928, NG_008401, NC_000017 |
| UniProt ID(s) | A7REI1, K7EMP8, P14136 |
| UniGene ID(s) | A7REI1, K7EMP8, P14136 |
| HGNC ID(s) | 4235 |
| Cosmic ID(s) | GFAP |
| KEGG Gene ID(s) | hsa:2670 |
| PharmGKB ID(s) | PA28647 |
| General Description of GFAP. | |
| This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte, neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain, for documenting astrocytic differentiation in tumors outside the CNS. | |
Limitations and Warranty
enQuire Bio’s GFAP Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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