PDF DatasheetHuman Anti-Glypican-3 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Glypican-3 Antibody Clone: 1G12
Clone 1G12 Host and Isotype: Mouse IgG1 kappa
Anti-Human Glypican-3 Positive Control Sample: 293T cells or Hepatocellular carcinoma
Cellular Localization of Antibody <1G12 Staining: Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A recombinant fragment containing amino acids 511-580 of human glypican-3
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Glypican-3 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-GPC3 antibody staining is expected to be primarily localized to the plasma membrane.
| Glypican-3 General Information | |
|---|---|
| Alternate Names | |
| Glypican-3, GPC3 | |
| Molecular Weight | |
| 67kDa | |
| Chromosomal Location | |
| Xq26.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GPC3 |
| Entrez Gene ID | 2719 |
| Ensemble Gene ID | ENSG00000147257 |
| RefSeq Protein Accession(s) | XP_016884902, NP_001158090, NP_001158089, NP_001158091, NP_004475 |
| RefSeq mRNA Accession(s) | XM_017029413, NM_001164618, NM_001164617, NM_004484 NM_001164619 |
| RefSeq Genomic Accession(s) | NC_018934, NG_009286, NC_000023 |
| UniProt ID(s) | Q53H15, B4DTD8, I6QTG3, P51654 |
| UniGene ID(s) | Q53H15, B4DTD8, I6QTG3, P51654 |
| HGNC ID(s) | 4451 |
| Cosmic ID(s) | GPC3 |
| KEGG Gene ID(s) | hsa:2719 |
| PharmGKB ID(s) | PA28832 |
| General Description of Glypican-3. | |
| Recognizes a protein, which is identified as HGAL. It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM),, two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4. HGAL is specifically expressed in germinal center B-cells, but is absent in mantle, marginal zone B-cells, in the inter-follicular, para-cortical regions in normal tonsils, lymph nodes. Its high degree of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas. HGAL expression has been used to help elucidate nodal marginal zone lymphoma (NMZL) from cases of diffuse follicle center lymphoma. Additionally, HGAL expression was shown to correlate with survival in patients with diffuse large B-cell lymphoma (DLBCL). | |
Limitations and Warranty
enQuire Bio’s Glypican-3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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