PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Polyclonal
Anti-Granzyme B Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide from the N-terminus of human granzyme B.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Granzyme B Information for Pathologists
Summary:
Enzyme associated with cytotoxic T lymphocytes; induces apoptosis in target cells of these lymphocytes.
| Granzyme B General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 27.7 kDa | |
| Chromosomal Location | |
| q12 [chr: 14] [chr_start: 24630954] [chr_end: 24634267] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GZMB |
| Entrez Gene ID | 3002 |
| RefSeq Protein Accession(s) | NP_004122 |
| RefSeq mRNA Accession(s) | NM_001346011; NR_144343; NM_004131; |
| RefSeq Genomic Accession(s) | NC_000014; NG_028340 |
| UniProt ID(s) | P10144 |
| KEGG Gene ID(s) | hsa:3002 |
| General Description of Granzyme B . | |
| This antibody reacts with a 32 kDa protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned. Human granzyme B is involved in target cell apoptosis during lymphocyte-mediated cytotoxicity. This antibody is useful in the localization of granzyme B containing lytic granules and for the characterization of activated CTL or NK cells. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
There are no reviews yet.