PDF DatasheetHuman Anti-IgM Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IgM Antibody Clone: ICO-30
Clone ICO-30 Host and Isotype: Mouse IgG1 kappa
Anti-Human IgM Positive Control Sample: 293T, Raji or hPBL cells. Tonsil or Spleen.
Cellular Localization of Antibody Cytoplasm, Cell Surface, Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Heavy chain of human IgM
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
IgM Previously Observed Antibody Staining Patterns
| IgM General Information | |
|---|---|
| Alternate Names | |
| Ig mu chain C region, IGHM | |
| Molecular Weight | |
| 50-75kDa | |
| Chromosomal Location | |
| 14q32.33 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IGHM |
| Entrez Gene ID | 3507 |
| RefSeq Genomic Accession(s) | NC_000014, NG_187600, NC_018925, NG_001019 |
| UniGene ID(s) | |
| HGNC ID(s) | 5541 |
| Cosmic ID(s) | IGHM |
| General Description of IgM. | |
| Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells, as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom’s macroglobulinemia, chronic infection, hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas,, certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal, therefore malignant. | |
Limitations and Warranty
enQuire Bio’s IgM Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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