PDF DatasheetMouse Anti-IL-4 / Interleukin-4 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-IL-4 / Interleukin-4 Antibody Clone: 11B11
Clone 11B11 Host and Isotype: Rat IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
IL-4 / Interleukin-4 Previously Observed Antibody Staining Patterns
| IL-4 / Interleukin-4 General Information | |
|---|---|
| Alternate Names | |
| IL4, Interleukin-4, BSF-1, BSF1, IL-4, BCGF1, BCGF-1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Il4 |
| Entrez Gene ID | 16189 |
| Ensemble Gene ID | ENSMUSG00000000869 |
| RefSeq Protein Accession(s) | NP_067258 |
| RefSeq mRNA Accession(s) | NM_021283, NR_027491 |
| RefSeq Genomic Accession(s) | NC_000077 |
| UniProt ID(s) | P07750 |
| UniGene ID(s) | P07750 |
| Cosmic ID(s) | Il4 |
| KEGG Gene ID(s) | mmu:16189 |
| General Description of IL-4 / Interleukin-4. | |
| The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions. Please choose the appropriate format for each application. | |
Selected References
Cook PC, Jones LH, Jenkins SJ, Wynn TA, Allen JE, and MacDonald AS. 2012. Proc. Natl. Acad. Sci. 109: 9977-9982. (in vivo blocking)Altin JA, Goodnow CC, and Cook MC. 2012. J. Immunol. 5478-5488. (Flow cytometry)Tofukuji S, Kuwahara M, Suzuki J, Ohara O, Nakayama T, and Yamashita M. 2012. J. Immunol. 188: 4846-4857. (in vitro Th1 polarization)Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846 (in vitro Th1 polarization, ELISA)Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy – frozen tissue, Immunohistochemistry – frozen tissue)
Limitations and Warranty
enQuire Bio’s Mouse Anti-IL-4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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