PDF DatasheetHuman and Mouse Anti-IL-6 / Interferon beta-2 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IL-6 / Interferon beta-2 Antibody Clone: IL6/1270
Clone IL6/1270 Host and Isotype: Rat IgG1 kappa
Anti-Human and Mouse IL-6 / Interferon beta-2 Positive Control Sample: Heart
Cellular Localization of Antibody Cytoplasmic, extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant IL-6 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
IL-6 / Interferon beta-2 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-IL6 antibody staining is expected to be primarily localized to the vesicles.
Observed Antibody Staining Data By Tissue Type:
Variations in IL-6 / Interferon beta-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of IL-6 / Interferon beta-2 could be seen inlymphoid tissue in appendix. We were unable to detect IL-6 / Interferon beta-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| IL-6 / Interferon beta-2 General Information | |
|---|---|
| Alternate Names | |
| Interleukin 6, IL-6, IL6 | |
| Molecular Weight | |
| 21kDa | |
| Chromosomal Location | |
| 7p21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IL6 |
| Entrez Gene ID | 3569 |
| Ensemble Gene ID | ENSG00000136244 |
| RefSeq Protein Accession(s) | XP_005249802, XP_011513692, NP_001305024, NP_000591 |
| RefSeq mRNA Accession(s) | XM_005249745, NM_000600, XM_011515390 NM_001318095 |
| RefSeq Genomic Accession(s) | NC_000007, NG_011640, NC_018918 |
| UniProt ID(s) | B4DVM1, P05231, Q75MH2, B4DNQ5, B5MC21 |
| UniGene ID(s) | B4DVM1, P05231, Q75MH2, B4DNQ5, B5MC21 |
| HGNC ID(s) | 6018 |
| Cosmic ID(s) | IL6 |
| KEGG Gene ID(s) | hsa:3569 |
| PharmGKB ID(s) | PA198 |
| General Description of IL-6 / Interferon beta-2. | |
| IL-6 is a potent lymphoid cell growth factor that stimulates the growth, survivability of certain B-cells, T-cells. It plays a critical role in B-cell differentiation to plasma cells, is a potent growth factor for plasmacytoma, myeloma. IL-6 is produced by a variety of cell types, including monocytes, fibroblasts, endothelial cells. Upon stimulation, macrophages, T, B, mast,, glial cells, eosinophils, keratinocytes, granulocytes also secrete IL-6. It is involved in host defense, acute phase reactions, immune responses,, hematopoiesis. | |
Limitations and Warranty
enQuire Bio’s IL-6 / Interferon beta-2 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.