PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Kappa Light Chain Antibody Clone: KP-53
Host and Isotype: Mouse IgG2b
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody KP-53 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with purified Bence Jones kappa light chain protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Kappa Light Chain Information for Pathologists
Summary:
Type of immunoglobulin light chain. Kappa/lamba ratio is usually 2:1. Restricted expression of either kappa or lambda suggests monoclonality and a neoplastic process. Microscopic (histologic) images Images hosted on Pathout server:.
| Kappa Light Chain General Information | |
|---|---|
| Alternate Names | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Multiple |
| General Description of Kappa Light Chain . | |
| This antibody reacts with B cell follicles in human lymphoid tissues. The mantle zones give a mosaic pattern, while the germinal centers show a coarse meshwork pattern of staining. This antibody stains free as well as bound kappa light chains. This antibody does not react with human lambda light chain. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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