PDF DatasheetMouse Anti-KLRG1 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-KLRG1 Antibody Clone: 2F1
Clone 2F1 Host and Isotype: Golden Syrian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
KLRG1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Staining with anti-KLRG1 antibody reveals KLRG1 expression is expected to be primarily localized to the vesicles.
| KLRG1 General Information | |
|---|---|
| Alternate Names | |
| MAFA-LIKE, MAFA-2F1, MAFA-L, 2F1, CLEC15A, MAFA, Killer cell lectin-like receptor subfamily G member | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Klrg1 |
| Entrez Gene ID | 50928 |
| Ensemble Gene ID | ENSMUSG00000030114 |
| RefSeq Protein Accession(s) | NP_058666 |
| RefSeq mRNA Accession(s) | NM_016970 |
| RefSeq Genomic Accession(s) | NC_000072 |
| UniProt ID(s) | O88713 |
| UniGene ID(s) | O88713 |
| Cosmic ID(s) | Klrg1 |
| KEGG Gene ID(s) | mmu:50928 |
| General Description of KLRG1. | |
| The 2F1 antibody reacts with mouse KLRG1 (Killer cell Lectin-like Receptor G1). This 30-38 kDa homodimeric receptor may be expressed by activated, mature NK cells and by effector/memory T cells, with potentially different roles in each cell type. KLRG1 can regulate, in an inhibitory fashion, the development and effector functions of NK cells, and is often cited as a senescence or terminal differentiation marker for T cells. Ligands for KLRG1 include members of the cadherin family of adhesion molecules, specifically N-Cadherin, E-Cadherin, and R-Cadherin. These interactions may induce bidirectional, immunosuppressive signaling in both KLRG- and Cadherin-expressing cells. A more recently identified role for KLRG1-Cadherin signaling in tissue organization, e.g. in cardiac angiogenesis, expands the function of these interactions beyond immunosuppression of immune cells. (Bouchentouf et al. 2010. J. Immunol. 185: 7014-7025).The 2F1 antibody may be used as a phenotypic marker for KLRG1 in mouse, frequently in combination with Mouse Anti-CD127 antibody (clone A7R34), for identification of effector T cell populations. | |
Selected References
Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).Tessmer MS, Fugere C, Stevenaert F, Naidenko OV, Chong HJ, Leclercq G, and Brossay L. 2007. Int. Immunol. 19:391-400. (Immunoprecipitation, in vitro blocking, Flow cytometry)Robbins SH, Nguyen KB, Takahashi N, Mikayama T, Biron CA, and Brossay L. 2002. J. Immunol. 168: 2585-2589. (in vitro blocking)Hanke T, Corral L, Vance RE, and Raulet DH. 1998. Eur. J. Immunol. 28(12): 4409-4417. (Origination of 2F1 clone)
Limitations and Warranty
enQuire Bio’s Mouse Anti-KLRG1 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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