PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:5-1:20 |
| Pretreatment | HistoZyme |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Laminin Information for Pathologists
Summary:
Major component on basement membrane, with type IV collagen. Trimeric glycoproteins (at least 11 types) with integrin binding site. Defects in laminin proteins cause skin blistering and myocardial diseases. Antibodies to laminin inhibit lung branching morphogenesis. Production of alpha-1 laminin by epithelial cells and mesenchymal cells depends on extent of mesenchymal and epithelial contact with each other; modulates mesenchymal cell shape.
Common Uses By Pathologists:
Double immunostaining with cytokeratin is useful to detect microinvasion in VIN or CIN (Arch Pathol Lab Med 2005;129:747).
| Laminin General Information | |
|---|---|
| Alternate Names | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Multiple |
| General Description of Laminin . | |
| This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment. | |
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