PDF DatasheetMouse Anti-Ly-6G Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Ly-6G Antibody Clone: 1A8
Clone 1A8 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Ly-6G Previously Observed Antibody Staining Patterns
| Ly-6G General Information | |
|---|---|
| Alternate Names | |
| Gr-1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ly6g |
| Entrez Gene ID | 546644 |
| Ensemble Gene ID | ENSMUSG00000022582 |
| RefSeq Protein Accession(s) | NP_001297367, XP_006521677 |
| RefSeq mRNA Accession(s) | NM_008531, NM_001310438, XM_006521614 |
| RefSeq Genomic Accession(s) | NC_000081, |
| UniProt ID(s) | A0A087WQF5 |
| Cosmic ID(s) | Ly6g |
| KEGG Gene ID(s) | mmu:546644 |
| General Description of Ly-6G. | |
| The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. | |
Selected References
Kamaly N, Fredman G, Subramanian M, Gadde S, Pesic A, Cheung L, Fayad ZA, Langer R, Tabas I and Cameron O. 2013. Proc. Natl. Acad. Sci. 110. 6506-6511. (flow cytometry). Stinn W, Buettner A, Weiler H, Friedrichs B, Luetjen S, van Overveld F, Meurrens K, Janssens K, Gebel S, Stabbert R and Haussman H-J. 2013. Toxicol. Sci. 131: 596-611 (flow cytometry).Enoksson M, Moller-Westerberg C, Wicher G, Fallon PG, Forsberg-Nilsson K, Lunderius-Andersson C and Nilsson G. 2013. Blood. 121. 530-536 (flow cytometry).
Limitations and Warranty
enQuire Bio’s Mouse Anti-Ly-6G Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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