Human Anti-MAML2 Antibody Product Attributes
MAML2 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MAML2 antibody staining is expected to be primarily localized to the nuclear bodies, nucleoplasm and cytosol. There is variability in either the signal strength or the localization of signal in nuclear bodies from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in MAML2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the tubules in kidney, glandular cells in the adrenal gland, colon, duodenum, prostate, salivary gland and stomach, Leydig cells in the testis, macrophages in lung, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus, squamous epithelial cells in the esophagus, oral mucosa and tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the tubules in kidney, glandular cells in the adrenal gland, colon, duodenum, prostate, salivary gland and stomach, Leydig cells in the testis, macrophages in lung, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus, squamous epithelial cells in the esophagus, oral mucosa and tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of MAML2 could be seen inadipocytes in breast, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil, glandular cells in the endometrium and epididymis, glial cells in the caudate nucleus, hematopoietic cells in the bone marrow, neuronal cells in the hippocampus and neuropil in cerebral cortex. We were unable to detect MAML2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of MAML2 expression as measured by anti-MAML2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | + | + | ++ | ++ | ++ | – | + | ++ | – | + | + | ++ | ++ | ++ | + | ++ | + | + | ++ | + |
MAML2 Variability | ++ | ++ | ++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ | ++ | + | +++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ |
MAML2 General Information | |
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Alternate Names | |
Mastermind-like protein 2, MAML2 | |
Molecular Weight | |
125kDa | |
Chromosomal Location | |
11q21 | |
Curated Database and Bioinformatic Data | |
Gene Symbol | MAML2 |
Entrez Gene ID | 84441 |
Ensemble Gene ID | ENSG00000184384 |
RefSeq Protein Accession(s) | XP_011541327, NP_115803, XP_011541326, XP_011541325 |
RefSeq mRNA Accession(s) | XM_011543024, XM_011543025, XM_011543023, NM_032427 |
RefSeq Genomic Accession(s) | NC_000011, NC_018922 |
UniProt ID(s) | Q8IZL2, Q6Y3A3, Q8IUL3 |
UniGene ID(s) | Q8IZL2, Q6Y3A3, Q8IUL3 |
HGNC ID(s) | 16259 |
Cosmic ID(s) | MAML2 |
KEGG Gene ID(s) | hsa:84441 |
PharmGKB ID(s) | PA134946327, PA30730 |
General Description of MAML2. | |
MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J? ;a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors. |
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