PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
MART-1 Information for Pathologists
Summary:
Melanocyte specific cytoplasmic protein involved in the formation of stage II melanosomes. MelanA was discovered as an antigen recognized by tumor infiltrating cytotoxic T cells from a melanoma patient, hence the name Melanoma Antigen (J Exp Med 1994;180:35). Same gene was independently identified by a different research group who named it MART1 (Melanoma Antigen Recognized by T cells 1) (Proc Natl Acad Sci USA 1994;91:3515); MelanA and MART1 are synonyms. Two antibodies are commercially available: A103 and M2-7C10. A103: mouse monoclonal antibody against MelanA recombinant protein (Proc Natl Acad Sci USA 1996;93:5915, US patent 5,674,749).
Common Uses By Pathologists:
To establish the diagnosis of metastatic melanoma, more sensitive than anti-gp100 antibody (HMB45) (Am J Surg Pathol 1998;22:976). Used in conjunction with S100 as a standard panel for evaluating sentinel lymph nodes for melanoma; HMB45 can be added to the panel but is not essential (Am J Surg Pathol 2001;25:1039). To confirm the diagnosis of angiomyolipoma, lymphangioleiomyomatosis (LAM) and other PEComas. To distinguish adrenal cortical tumors from renal cell carcinoma (clone A103 only); inhibin A can also be used for this purpose. To confirm the diagnosis of a sex cord stromal tumor (clone A103 only); inhibin A can also be used for this purpose.
| MART-1 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 13.2 kDa | |
| Chromosomal Location | |
| p24.1 [chr: 9] [chr_start: 5890802] [chr_end: 5910606] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MLANA |
| Entrez Gene ID | 2315 |
| RefSeq Protein Accession(s) | NP_005502 |
| RefSeq mRNA Accession(s) | NM_005511 |
| RefSeq Genomic Accession(s) | NC_000009 |
| UniProt ID(s) | Q16655 |
| PharmGKB ID(s) | PA30842 |
| KEGG Gene ID(s) | hsa:2315 |
| General Description of MART-1 . | |
| This product is intended for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissue sections, to be viewed by light microscopy. Clinical interpretation of staining results should be accompanied by histological studies with proper controls. Patients’ clinical histories and other relevant diagnostic tests should be utilized by a qualified person(s) when evaluating and interpreting results. | |
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