PDF DatasheetHuman, Mouse, Rat, and Canine Anti-MART-1 / Melan-A / MLANA Antibody Product Attributes
Species: Human, Mouse, Rat, and Canine
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MART-1 / Melan-A / MLANA Antibody Clone: A103
Clone A103 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, Rat, and Canine MART-1 / Melan-A / MLANA Positive Control Sample: SK-MEL-13, SK-MEL-19 Melanoma cell lines; Melanomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant hMART-1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
MART-1 / Melan-A / MLANA Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in MART-1 / Melan-A / MLANA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in melanocytes in skin. More moderate antibody staining intensity was present in melanocytes in skin. Low, but measureable presence of MART-1 / Melan-A / MLANA could be seen in. We were unable to detect MART-1 / Melan-A / MLANA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| MART-1 / Melan-A / MLANA General Information | |
|---|---|
| Alternate Names | |
| Protein melan-A melanoma antigen recognized by T cells 1, MART-1, MLANA | |
| Molecular Weight | |
| 20-22kDa (doublet) | |
| Chromosomal Location | |
| 9p24.1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MLANA |
| Entrez Gene ID | 2315 |
| Ensemble Gene ID | ENSG00000120215 |
| RefSeq Protein Accession(s) | NP_005502 |
| RefSeq mRNA Accession(s) | NM_005511 |
| RefSeq Genomic Accession(s) | NC_018920, NC_000009 |
| UniProt ID(s) | Q16655 |
| UniGene ID(s) | Q16655 |
| HGNC ID(s) | 7124 |
| Cosmic ID(s) | MLANA |
| KEGG Gene ID(s) | hsa:2315 |
| PharmGKB ID(s) | PA30842 |
| General Description of MART-1 / Melan-A / MLANA. | |
| This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1,, GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes, endoplasmic reticulum. This MAb labels melanomas, other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. | |
Limitations and Warranty
enQuire Bio’s MART-1 / Melan-A / MLANA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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