PDF DatasheetHuman Anti-Melanoma Marker Antibody Product Attributes
Species: Human
Tested Applications: Immunohistochemistry (IHC).
Application Notes: Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Melanoma Marker Antibody Clone: M2-7C10 + M2-9E3 + HMB45 + T311
Clone M2-7C10 + M2-9E3 + HMB45 + T311 Host and Isotype: Mouse IgG1 IgG2a & IgG2b kappa
Anti-Human Melanoma Marker Positive Control Sample: SK-MEL-13, SK-MEL-19 Melanoma cell lines; Melanomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA and Azide under Cat # MSM1-701-P1ABX.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant hMART-1 protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Melanoma Marker Previously Observed Antibody Staining Patterns
| Melanoma Marker General Information | |
|---|---|
| Alternate Names | |
| Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17, anti-Melanoma antigen recognized by T-cells 1 (MART-1) antibody, anti-MLAN-A antibody, anti-TYR antibody, anti-PMEL17 antibody | |
| Molecular Weight | |
| 20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 90-100kDa (gp100) | |
| Chromosomal Location | |
| Multiple | |
| Curated Database and Bioinformatic Data | |
| Entrez Gene ID | , 7299, 6490 |
| Ensemble Gene ID | , ENSG00000077498, ENSG00000185664 |
| RefSeq Protein Accession(s) | , XP_011541272, NP_000363, NP_001186982, XP_006719632, NP_001307050, NP_008859, XP_011536987, NP_001186983, NP_001307051 |
| RefSeq mRNA Accession(s) | , NM_000372, XM_011542970, , XM_006719569, NM_001320122, NM_001200053, XM_011538685, NM_001320121, NM_006928 NM_001200054 |
| RefSeq Genomic Accession(s) | , NC_000011, NG_008748, NC_018922, NC_000012, NC_018923, NG_028086 |
| UniProt ID(s) | , P14679, L8B082, P40967 |
| UniGene ID(s) | , P14679, L8B082, P40967 |
| HGNC ID(s) | , 12442, 10880 |
| Cosmic ID(s) | , TYR, PMEL |
| KEGG Gene ID(s) | , hsa:7299, hsa:6490 |
| PharmGKB ID(s) | , PA37095, PA35781 |
| General Description of Melanoma Marker. | |
| This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase, gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes, melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas, other tumors showing melanocytic differentiation. | |
Limitations and Warranty
enQuire Bio’s Melanoma Marker Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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