PDF DatasheetHuman Anti-Milk Fat Globule Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Milk Fat Globule Antibody Clone: EDM45
Clone EDM45 Host and Isotype: Mouse IgG1 kappa
Anti-Human Milk Fat Globule Positive Control Sample: Normal breast or breast carcinoma
Cellular Localization of Antibody Cell Surface & Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Delipidated human milk fat globule membrane preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Milk Fat Globule Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MFGE8 antibody staining is expected to be primarily localized to the cytosol.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Milk Fat Globule expression as measured by anti-Milk Fat Globule antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | +++ | ++ | +++ | ++ | +++ | – | +++ | ++ | +++ | – | – | +++ | +++ | – | +++ | – | +++ | + | + | +++ |
| MFGE8 Variability | + | +++ | + | ++ | + | ++ | ++ | ++ | ++ | + | ++ | + | + | ++ | + | ++ | ++ | ++ | ++ | + |
| Milk Fat Globule General Information | |
|---|---|
| Alternate Names | |
| Milk fat globule-EGF factor 8 protein, Mfge8, lactadherin | |
| Molecular Weight | |
| 45kDa | |
| Chromosomal Location | |
| 15q25 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MFGE8 |
| Entrez Gene ID | 4240 |
| Ensemble Gene ID | ENSG00000140545 |
| RefSeq Protein Accession(s) | NP_001108086, XP_016877695, NP_005919, NP_001297249, NP_001297248, NP_001297250 |
| RefSeq mRNA Accession(s) | NM_001310319, XM_017022206, NM_001114614, XR_931838, NM_001310321, NM_005928, NM_001310320 |
| RefSeq Genomic Accession(s) | NC_000015, NC_018926 |
| UniProt ID(s) | B3KTQ2, X6R3G6, Q08431, B4E396, F5GZN3 |
| UniGene ID(s) | B3KTQ2, X6R3G6, Q08431, B4E396, F5GZN3 |
| HGNC ID(s) | 7036 |
| Cosmic ID(s) | MFGE8 |
| KEGG Gene ID(s) | hsa:4240 |
| PharmGKB ID(s) | PA30772 |
| General Description of Milk Fat Globule. | |
| Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells, cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker, can also provide a tool to study the role of the cell surface in normal, neoplastic mammary development. | |
Limitations and Warranty
enQuire Bio’s Milk Fat Globule Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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