PDF DatasheetHuman Anti-MUC3 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MUC3 Antibody Clone: SPM200
Clone SPM200 Host and Isotype: Mouse IgG2a kappa
Anti-Human MUC3 Positive Control Sample: LS174T cells or colon carcinoma, normal colon tissue.
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
MUC3 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of MUC3 expression as measured by anti-MUC3 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | + | – | + | – | – | – | + | – | – | – | – | – | + | + | – | – | – | + | – |
| MUC3A Variability | + | +++ | ++ | ++ | ++ | + | + | ++ | + | + | ++ | ++ | + | ++ | ++ | + | + | ++ | ++ | + |
| MUC3 General Information | |
|---|---|
| Alternate Names | |
| Mucin 3A, MUC3A | |
| Molecular Weight | |
| 1100kDa | |
| Chromosomal Location | |
| 7q22.1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MUC3A |
| Entrez Gene ID | 4584, 57876 |
| Ensemble Gene ID | ENSG00000169894 |
| RefSeq Protein Accession(s) | XP_011514549, XP_011514551, XP_016867720, XP_011514552, XP_011514548, NP_005951 |
| RefSeq mRNA Accession(s) | XM_011516246, XM_011516247, XM_011516250, XM_017012231, XM_011516249, NM_005960 |
| RefSeq Genomic Accession(s) | NC_018918, NC_000007 |
| UniProt ID(s) | Q9UN95, Q02505, Q9H3Q6 |
| UniGene ID(s) | Q9UN95, Q02505, Q9H3Q6 |
| HGNC ID(s) | 7513, 13384 |
| Cosmic ID(s) | MUC3A, MUC3B |
| KEGG Gene ID(s) | hsa:4584 |
| General Description of MUC3. | |
| It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon, rectum,, is also present to a lesser extent in breast, lung, salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2), 51 base pairs (Mucin 3). The number of repeats is highly polymorphic, varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines, airways, is associated with colonic tumors. Mucin 3 is a major component of various mucus gels, is broadly expressed in normal, tumor cells. | |
Limitations and Warranty
enQuire Bio’s MUC3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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