PDF DatasheetHuman, Mouse, Rat, Chicken, and Pig Anti-Neurofilament Antibody Product Attributes
Species: Human, Mouse, Rat, Chicken, and Pig
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Neurofilament Antibody Clone: NF421 + NFL/736
Clone NF421 + NFL/736 Host and Isotype: Mouse IgG
Anti-Human, Mouse, Rat, Chicken, and Pig Neurofilament Positive Control Sample: Brain, Neuroblastoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human neurofilament protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Neurofilament Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-NEFH antibody staining is expected to be primarily localized to the centrosome, nuclear bodies and nucleus.
| Neurofilament General Information | |
|---|---|
| Alternate Names | |
| NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 2kDa, Neurofilament Triplet H Protein, NF-H, NF2. 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68, anti-NEFH antibody, anti-Neurofilament H antibody, anti-Neurofilament Heavy Polypeptide 2kDa antibody, anti-Neurofilament Triplet H Protein antibody, anti-NF-H antibody, anti-NF2. 68kDa neurofilament protein antibody, anti-Light molecular weight neurofilament protein antibody, anti-NEFL antibody, anti-Neurofilament light polypeptide 68kDa antibody, anti-Neurofilament light polypeptide antibody, anti-Neurofilament protein antibody, anti-light chain antibody, anti-Neurofilament subunit NF-L antibody, anti-Neurofilament triplet L protein antibody, anti-NF-L antibody, anti-NF68 antibody | |
| Molecular Weight | |
| 200kDa & 68kDa | |
| Chromosomal Location | |
| 22q12.2 (NEFH) & 8p21.2 (NEFL) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NEFH & NEFL |
| Entrez Gene ID | 4744 |
| Ensemble Gene ID | ENSG00000100285 |
| RefSeq Protein Accession(s) | XP_011528502, NP_066554 |
| RefSeq mRNA Accession(s) | NM_021076, XM_011530200 |
| RefSeq Genomic Accession(s) | NG_008404, NC_018933, NC_000022 |
| UniProt ID(s) | P12036 |
| UniGene ID(s) | P12036 |
| HGNC ID(s) | 7737 |
| Cosmic ID(s) | NEFH |
| KEGG Gene ID(s) | hsa:4744 |
| PharmGKB ID(s) | PA31540 |
| General Description of Neurofilament. | |
| This MAb reacts with a 200kDa, 68kDa protein, identified as heavy, light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons, dendrites, are found in neurons, peripheral nerves,, sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M), 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine,, endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas,, neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal, extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin,, oat cell carcinomas of the lung also express neurofilament. | |
Limitations and Warranty
enQuire Bio’s Neurofilament Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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