PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Neuron Specific Enolase Information for Pathologists
Summary:
Cytoplasmic enzyme expressed by neuroendocrine cells and tumors, including poorly differentiated neuroendocrine tumors, which usually are negative for other neuroendocrine markers. Despite its name, is often not specific for neuroendocrine cells / tumors. Enolases are widely distributed glycolytic enzymes that catalyze the interconversion of 2-phosphoglycerate and phosphophenolpyruvate. NSE refers to the gamma-gamma and alpha-gamma isoenzymes preferentially found in neurons and neuroendocrine cells. Microscopic (histologic) images
| Neuron Specific Enolase General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 47.3 kDa | |
| Chromosomal Location | |
| p13.31 [chr: 12] [chr_start: 6913745] [chr_end: 6923698] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ENO2 |
| Entrez Gene ID | 2026 |
| RefSeq Protein Accession(s) | NP_001966 |
| RefSeq mRNA Accession(s) | NM_001975 |
| RefSeq Genomic Accession(s) | NC_000012 |
| UniProt ID(s) | P09104 |
| PharmGKB ID(s) | PA27788 |
| KEGG Gene ID(s) | hsa:2026 |
| General Description of Neuron Specific Enolase . | |
| This antibody is specific to γ-γ enolase isozyme of 46 kDa. This antibody stains neurons, neuroendocrine cells, gangliomas, oat cell carcinomas, astrocytomas, paragangliomas, glioblastomas, pheochromocytomas, melanomas and schwannomas. | |
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