Anti-Nucleolin Antibody Clone 364-5

$ 488.00

SKU: 4691-MSM2
Species: Human (-), Mouse (-), Rat (-), and Cow (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Human (-), Mouse (-), Rat (-), and Cow (-) Anti-Nucleolin Antibody Product Attributes

Species: Human (-), Mouse (-), Rat (-), and Cow (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Nucleolin Antibody Clone: 364-5
Clone 364-5 Host and Isotype: Mouse IgG1 kappa
Anti-Human (-), Mouse (-), Rat (-), and Cow (-) Nucleolin Positive Control Sample: All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin’s lymphoma
Cellular Localization of Antibody <364-5 Staining: Nucleoli
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Lysate of SU-DHL-1 Nuclei
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Nucleolin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NCL antibody staining is expected to be primarily localized to the nucleoli and nucleus.

Observed Antibody Staining Data By Tissue Type:

Variations in Nucleolin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland and appendix, lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, glandular cells in the breast, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, endothelial cells in the colon, glandular cells in the colon, peripheral nerve/ganglion in colon, glandular cells in the duodenum, cells in the endometrial stroma in endometrium, glandular cells in the endometrium and epididymis, squamous epithelial cells in the esophagus, glandular cells in the fallopian tube and gallbladder, myocytes in heart muscle, glial cells in the hippocampus, neuronal cells in the hippocampus, cells in the glomeruli in kidney, cells in the tubules in kidney, bile duct cells in the liver, hepatocytes in liver, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, follicle cells in the ovary, ovarian stroma cells in the ovary, exocrine glandular cells in the pancreas, islets of Langerhans in pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland and seminal vesicle, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, non-germinal center cells in the tonsil, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder, squamous epithelial cells in the vagina, cells in the red pulp in spleen, cells in the white pulp in spleen, glandular cells in the stomach, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland and germinal center cells in the tonsil. More moderate antibody staining intensity was present in glandular cells in the adrenal gland and appendix, lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, glandular cells in the breast, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, endothelial cells in the colon, glandular cells in the colon, peripheral nerve/ganglion in colon, glandular cells in the duodenum, cells in the endometrial stroma in endometrium, glandular cells in the endometrium and epididymis, squamous epithelial cells in the esophagus, glandular cells in the fallopian tube and gallbladder, myocytes in heart muscle, glial cells in the hippocampus, neuronal cells in the hippocampus, cells in the glomeruli in kidney, cells in the tubules in kidney, bile duct cells in the liver, hepatocytes in liver, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, follicle cells in the ovary, ovarian stroma cells in the ovary, exocrine glandular cells in the pancreas, islets of Langerhans in pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland and seminal vesicle, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, non-germinal center cells in the tonsil, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder, squamous epithelial cells in the vagina, cells in the red pulp in spleen, cells in the white pulp in spleen, glandular cells in the stomach, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland and germinal center cells in the tonsil. Low, but measureable presence of Nucleolin could be seen in. We were unable to detect Nucleolin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio’s Nucleolin Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-Nucleolin Antibody Clone 364-5

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