PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
p120 Catenin Information for Pathologists
Summary:
p120 catenin is one of the transmembrane components of epithelial adherens junction proteins (J Cell Sci 2001;114:503), which includes p120 catenin and E-cadherin (Prog Mol Biol Transl Sci 2013;116:71). In the cadherin – catenin complex, which is essential for the formation of intercellular tight junctions, p120 catenin attaches to E-cadherin on the internal aspect of the cell membrane. p120 is composed of 2 domains, an external calcium dependent domain and an internal domain to which p120 catenin is bound (Oncogene 1996;13:1919) in a juxtamembranous location (J Cell Biol 1998;141:779). p120 is involved in promotion of cell junction formation, cell motility and E-cadherin trafficking and turnover. Binding of p120 to E-cadherin stabilizes the complex and increases the half-life of E-cadherin.
Common Uses By Pathologists:
Differentiation between ductal and lobular carcinoma / in situ lesions. p120 shows a membranous pattern of staining in ductal carcinoma and in situ lesions. Lack of E-cadherin staining and strong p120 cytoplasmic staining is feature of the spectrum of lobular lesions (atypical lobular hyperplasia (ALH), lobular carcinoma in situ (LCIS), lobular carcinoma). Positive cytoplasmic staining for lobular carcinoma is much easier to interpret than E-cadherin negative staining. E-cadherin and p120 catenin cocktail improve the diagnosis of in situ lesions (Int J Clin Exp Pathol 2014;7:2551).
| p120 Catenin General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 108.2 kDa | |
| Chromosomal Location | |
| q12.1 [chr: 11] [chr_start: 57753243] [chr_end: 57819546] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CTNND1 |
| Entrez Gene ID | 1500 |
| RefSeq Protein Accession(s) | NP_001078936; NP_001193818; NP_001322; NP_001078934; NP_001193819; NP_001078935; NP_001078928; NP_001193812; NP_001193816; NP_001193817; NP_001078932; NP_001193814; NP_001078927; NP_001078930; NP_001078937; NP_001078938; NP_001193815; NP_001078929; NP_001078931; NP_001078933; NP_001193813; NP_001193820 |
| RefSeq mRNA Accession(s) | NM_001085466; NM_001206888; NM_001206889; NM_001085459; NM_001206885; NM_001206886; NM_001206887; NM_001085465; NM_001206890; NM_001085458; NM_001085467; NM_001206883; NM_001331; NM_001085460; NM_001085461; NM_001085463; NM_001085468; NM_001085469; NM_001206884; NM_001085462; NM_001085464; NM_001206891 |
| RefSeq Genomic Accession(s) | NG_029078; NC_000011 |
| UniProt ID(s) | O60716 |
| PharmGKB ID(s) | PA27016 |
| KEGG Gene ID(s) | hsa:1500 |
| Associated Diseases (KEGG IDs) | Blepharocheilodontic syndrome 2 (BCDS2) [MIM:617681]: A form of blepharocheilodontic syndrome, a rare autosomal dominant disorder. It is characterized by lower eyelid ectropion, upper eyelid distichiasis, euryblepharon, bilateral cleft lip and palate, and features of ectodermal dysplasia, including hair anomalies, conical teeth and tooth agenesis. An additional rare manifestation is imperforate anus. There is considerable phenotypic variability among affected individuals. {ECO:0000269|PubMed:28301459}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of p120 Catenin . | |
| Cytoplasmic accumulation of p120 Catenin has been observed in lung cancer, pancreatic cancer, gastric cancer and colon cancers and is associated with poor progress in colon cancer patients. In breast lobular neoplasia, anti p120 Catenin shows a diffuse cytoplasmic immunostaining pattern,while breast ductal neoplasia retains the membrane immunostaining pattern. p120 Catenin antibody is useful in differentiation of lobular carcinoma from ductal carcinoma of the breast and in identifying early lesions of lobular neoplasia.rn | |
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