.jpg "p21WAF1 IHC human Colon Carcinoma (DCS-60.2)")
![Analysis of Mass Spec data (dashed-line) of fractions stained with p21WAF1 MS-QAVA™ monoclonal antibody [Clone: DCS-60.2] (solid-line), reveals that less than 14% of signal is attributable to non-specific binding of anti-p21WAF1 [Clone DCS-60.2] to targets other than CDKN1A protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-1026-MSM2-P1-anti-p21WAF1-antibody.png "p21WAF1 Antibody Mass Spec Validation Data")
PDF DatasheetHuman, Mouse (-), and Rat (-) Anti-p21WAF1 Antibody Product Attributes
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-p21WAF1 Antibody Clone: DCS-60.2
Clone DCS-60.2 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Mouse (-), and Rat (-) p21WAF1 Positive Control Sample: HeLa Cells. Skin, colon, or breast carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human recombinant p21 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
p21WAF1 Previously Observed Antibody Staining Patterns
| p21WAF1 General Information | |
|---|---|
| Alternate Names | |
| cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, p21Cip1, p21 Cip1, p21Waf1, p21 Waf1 | |
| Molecular Weight | |
| 21kDa | |
| Chromosomal Location | |
| 6p21.31 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDKN1A |
| Entrez Gene ID | 1026 |
| Ensemble Gene ID | ENSG00000124762 |
| RefSeq Protein Accession(s) | NP_001207706, NP_000380, NP_001207707, NP_001278478, NP_510867 |
| RefSeq mRNA Accession(s) | NM_001291549, NM_001220778, NM_000389, NM_078467, NM_001220777 |
| RefSeq Genomic Accession(s) | NG_009364, NC_000006, NC_018917 |
| UniProt ID(s) | A0A024RCX5, P38936 |
| UniGene ID(s) | A0A024RCX5, P38936 |
| HGNC ID(s) | 1784 |
| Cosmic ID(s) | CDKN1A |
| KEGG Gene ID(s) | hsa:1026 |
| PharmGKB ID(s) | PA104 |
| General Description of p21WAF1. | |
| This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21, shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s, a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase, is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). | |
Limitations and Warranty
This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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